Synthesis of ethyl ferulate in organic medium using celite-immobilized lipase

被引：53

作者：

Kumar, Ashok ^{[1
]}

Kanwar, Shamsher S. ^{[1
]}

机构：

[1] Himachal Pradesh Univ, Dept Biotechnol, Shimla 171005, India

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 03期

关键词：

Celite-545; Immobilization; Esterification; Ethyl ferulate; Molecular sieves; ACID; ESTERIFICATION; PURIFICATION;

D O I：

10.1016/j.biortech.2010.10.027

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In the present work we have evaluated synthesis of ethyl ferulate by the esterification reaction of ferulic acid and ethanol catalyzed by a commercial lipase (Steapsin) immobilized onto celite-545 in a short period of 6 h in DMSO. The immobilized lipase was treated with cross-linking agent glutaraldehyde (1%; v/v). The optimum synthesis of ethyl ferulate was recorded at 45 degrees C, pH 8.5 and 1:1 ratio of ethanol and ferulic acid. Co2+, Ba(2+)and Pb2+ ions enhanced the synthesis of ethyl ferulate Hg2+, Cd(3+)and NH4+ ions had mild inhibitory effect. The celite-bound lipase produced 68 mM of ethyl ferulate under optimized reaction conditions. (C) 2010 Elsevier Ltd. All rights reserved.

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页码：2162 / 2167

页数：6

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