Runx2, a target gene for activating transcription factor-3 in human breast cancer cells

被引：33

作者：

Gokulnath, M. ^{[1
]}

Partridge, N. C. ^{[2
]}

Selvamurugan, N. ^{[1
]}

机构：

[1] SRM Univ, Sch Bioengn, Dept Biotechnol, Kattankulathur 603203, Tamil Nadu, India

[2] NYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY 10010 USA

来源：

TUMOR BIOLOGY | 2015年 / 36卷 / 03期

关键词：

ATF-3; TGF-beta; 1; MMP-13; Runx2; Bone metastasis; ADAPTIVE-RESPONSE; FACTOR ATF3; A-GENE; EXPRESSION; DIFFERENTIATION; TUMOR; COLLAGENASE-3; METASTASIS; INHIBITION; BIOLOGY;

D O I：

10.1007/s13277-014-2796-x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Activating transcription factor (ATF-3) is a stress response gene and is induced by transforming growth factor beta 1 (TGF-beta 1) in breast cancer cells. In this study, we dissected the functional role of ATF-3 gene in vitro by knocking down its expression stably in human bone metastatic breast cancer cells (MDA-MB231). Knockdown of ATF-3 expression in these cells decreased cell number, altered cell cycle phase transition, and decreased mRNA expression of cell cycle genes. Knockdown of ATF-3 expression in MDA-MB231 cells also decreased cell migration, and the expression levels of invasive and metastatic genes such as MMP-13 and Runx2 were found to be decreased in these cells. Most importantly, ATF-3 was associated with Runx2 promoter in MDA-MB231 cells and knockdown of ATF-3 expression decreased its association with Runx2 promoter. Hence, our results suggested that ATF-3 plays a role in proliferation and invasion of bone metastatic breast cancer cells in vitro and we identified for the first time that Runx2 is a target gene of ATF-3 in MDA-MB231 cell line.

引用

页码：1923 / 1931

页数：9

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