IMMUNOHISTOCHEMICAL DETECTION OF ARTERIOLAR HYPERPLASIA IN CANINE LIVER BIOPSY SAMPLES USING THE CLAUDIN-5 ANTIBODY

被引：0

作者：

Jakab, Csaba ^{[1
]}

Rusvai, Miklos ^{[1
]}

Galfi, Peter ^{[2
]}

Szabara, Agnes ^{[1
]}

Szabo, Zoltan ^{[4
]}

Kulka, Janina ^{[3
]}

机构：

[1] Dept Pathol & Forens Vet Med, H-1078 Budapest, Hungary

[2] Dept Pharmacol & Toxicol, H-1078 Budapest, Hungary

[3] Semmelweis Univ, Dept Pathol 2, H-1085 Budapest, Hungary

[4] CJ Hall Vet Surg Exot Ctr, London, England

来源：

ACTA VETERINARIA HUNGARICA | 2010年 / 58卷 / 04期

关键词：

Arteriolar hyperplasia; dog; liver; biopsy; claudin-5; immunohistochemistry;

D O I：

10.1556/AVet.58.2010.4.3

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Claudins are key tight junctional proteins between adjacent epithelial, mesothelial or endothelial cells, which are responsible for the permeability of the paracellular space. This paper describes that the endothelial cells of normal hepatic arterioles, portal venules and portal lymphatics as well as the endothelium of sinusoids from dogs show strong membranous claudin-5 cross-reactivity. In 25 liver biopsy samples taken from dogs with portal vein hypoperfusion, an increased number of arterioles was detected in the portal areas (PAs) by the use of humanised anti-claudin-5 antibody. The increased number of hyperplastic hepatic arterioles per PA was 5-6, 8-12 and 15-20 in the case of small, medium-sized and large PAs, respectively. It is suggested that the claudin-5 marker can improve the detection of hepatic arteriolar proliferation in the PAs of liver samples.

引用

页码：423 / 430

页数：8