Kcc4 associates with septin proteins of Saccharomyces cerevisiae

Kcc4, a kinase of the budding yeast Saccharomyces cerevisiae, is homologous to the bud neck protein kinases Hsl1/ Nik1 and Gin4. We report here that a GFP-Kcc4 fusion protein is localized at the bud neck and that the non-kinase domain is required for this localization. We also demonstrate that Kcc4 associates with septin proteins in vitro and in vivo by two-hybrid analysis, GST pull-down experiments, immunoprecipitation, and analysis of direct association with affinity-purified GST-Kcc4 and MBP-Septin proteins, From the results obtained here, we suggest that Cdc11 is the primary association partner of Kcc4. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. AIl rights reserved.