Effects of fluoxetine on behavior, antioxidant enzyme systems, and multixenobiotic resistance in the Asian clam Corbicula fluminea

被引:67
作者
Chen, Huihui [1 ]
Zha, Jinmiao [1 ]
Yuan, Lilai [1 ]
Wang, Zijian [1 ]
机构
[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China
基金
中国国家自然科学基金;
关键词
Fluoxetine; C; fluminea; Siphoning behavior; Antioxidant enzymes; Multixenobiotic resistance; MUSSEL MYTILUS-GALLOPROVINCIALIS; FRESH-WATER MUSSELS; HUMAN PHARMACEUTICALS; DREISSENA-POLYMORPHA; ZEBRA MUSSEL; DRUG CARBAMAZEPINE; MASS-SPECTROMETRY; GREAT-LAKES; EXPOSURE; STRESS;
D O I
10.1016/j.chemosphere.2014.08.062
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Fluoxetine (FLX), a selective serotonin reuptake inhibitor, is widespread in aquatic environments. Despite its reported effects on behavior and reproduction in aquatic species, little is known about the effects of FLX on cellular detoxification and defense system in bivalves. Here, the adult Asian clam (Corbicula fluminea) was exposed to 0.5, 5, and 50 mu g L-1 FLX for 30 d. Siphoning behavior was inhibited by treatment with 50 mu g L-1 FLX. Additionally, superoxide dismutase (SOD) activity in the gills and digestive glands significantly decreased (p < 0.05) with 5 and 50 mu g L-1 FLX treatments, whereas catalase (CAT) activity and malondialdehyde (MDA) content markedly increased (p < 0.05). Moreover, transcription of thioredoxin reductase (TR), glutathione peroxidase (GPx) and Glutathione S-transferase pi class (GSTpi) was significantly upregulated (p < 0.05), whereas glutathione reductase (GR) was markedly downregulated (p < 0.05). These findings suggest that FLX affects behavior and induces oxidative stress in C. fluminea. The downregulation of ATP-binding cassette (ABC) transportor genes (ABCB1, ABCC1 and ABCG2) transporter genes indicated that FLX might suppress the multixenobiotic resistance (MXR) system in C. fluminea. Our results provide new insights into the adverse effects of FLX on the cellular detoxification and MXR system of C fluminea. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:856 / 862
页数:7
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