Is Relative Quantification Dispensable for the Measurement of MicroRNAs as Serum Biomarkers in Germ Cell Tumors?

被引：0

作者：

Spiekermann, Meike ^{[1
]}

Dieckmann, Klaus-Peter ^{[2
]}

Balks, Thomas ^{[2
]}

Bullerdiek, Joern ^{[1
,3
]}

Belge, Gazanfer ^{[1
]}

机构：

[1] Univ Bremen, Ctr Human Genet, D-28359 Bremen, Germany

[2] Albertinen Hosp, Dept Urol, Hamburg, Germany

[3] Univ Rostock, Inst Med Genet, D-18055 Rostock, Germany

来源：

ANTICANCER RESEARCH | 2015年 / 35卷 / 01期

关键词：

Biomarker; endogenous control; germ cell tumors; microRNA; real time PCR; testicular cancer; EXPRESSION; CANCER; RNA; IDENTIFICATION; MIR-371-3; DIAGNOSIS; MARKERS; PCR;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Classical biomarkers alpha-fetoprotein, beta-human chorionic gonadotropin and lactate dehydrogenase (AFP, bHCG and LDH) are elevated in only 60% of all testicular germ cell tumor (TGCT) patients. microRNAs (miRNAs) are a novel class of useful biomarkers in cancer and miRNAs of the miR-371-3 cluster were proven to be valuable markers for TGCT patients. Materials and Methods: We compared the Ct and Delta Ct values of miR-371-3 by real time PCR (qPCR) with and without 18S rRNA for normalization. Expression of miR-371a-3p, miR-372 and miR-373-3p was measured in 25 TGCTs, 4 non-TGCTs and 17 age-matched male controls. Results: A highly positive correlation between Ct and Delta Ct values was found in all samples. The highest correlation was found for miR-371a-3p (R2: 0.956). Conclusion: Results show that qPCR can be used without endogenous control for analyzing miR-371-3 in the serum of patients with testicular cancer and male controls if the technical procedure is performed under controlled conditions.

引用

页码：117 / 121

页数：5

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