Thermodynamic properties of DNA-dendrimer complexes and features of their applications

被引：2

作者：

Mdzinarashvili, Tamaz ^{[1
,2
]}

Khvedelidze, Mariam ^{[1
,2
]}

Shekiladze, Eka ^{[1
,2
]}

Shengelia, Nino ^{[1
,2
]}

Hianik, Tibor ^{[3
]}

机构：

[1] I Javakhishvili Tbilisi State Univ, Fac Exact & Nat Sci, 3 Chavchavadze Ave, GE-0128 Tbilisi, Georgia

[2] I Javakhishvili Tbilisi State Univ, Inst Med & Appl Biophys, 3 Chavchavadze Ave, GE-0128 Tbilisi, Georgia

[3] Comenius Univ, Fac Math Phys & Informat, Mlynska Dolina F1, Bratislava 84248, Slovakia

来源：

GENERAL PHYSIOLOGY AND BIOPHYSICS | 2018年 / 37卷 / 05期

关键词：

PAMAM dendrimers; Methanol; DNA; Gene delivery; Differential scanning calorimetry;

D O I：

10.4149/gpb_2018031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The commercial solution of fourth generation (G4) of poly-amidoamine (PAMAM) dendrimers contains methanol, which is toxic for human body. Our differential scanning calorimetry (DSC) study of dendrimers confirmed the existence of this threat. The recommendation is done on how to prepare dendrimer solutions for practical and safely use in gene delivery. DSC have been also used to study the thermodynamic properties of DNA/dendrimer complexes (dendriplexes). We showed that up to DNA/dendrimer ratio 43 +/- 3 (w/w) the solution was homogeneous, but stable aggregates were formed at higher PAMAM content. DSC experiments performed with homogeneous solution of dendriplexes revealed existence of the pH-dependent melting curves that contain several endothermic peaks associated with melting of GC-rich regions.

引用

页码：597 / 601

页数：5

共 15 条

[1] PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy [J].