An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

被引：14

作者：

Dulwich, Katherine L. ^{[1
]}

Asfor, Amin S. ^{[1
]}

Gray, Alice G. ^{[1
]}

Nair, Venugopal ^{[1
]}

Broadbent, Andrew J. ^{[1
]}

机构：

[1] Pirbright Inst, Guildford, Surrey, England

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2018年 / 140期

基金：

英国国家替代、减少和改良动物研究中心; 英国生物技术与生命科学研究理事会;

关键词：

Immunology and Infection; Issue; 140; Chicken; primary cells; bursa of Fabricius; B cells; virus; infectious bursa! disease virus; IBDV; CD40; LIGAND; IN-VITRO; B-CELLS; EXPRESSION; PROFILES; REVEALS; STRAINS; GENES;

D O I：

10.3791/58489

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Infectious bursal disease virus (IBDV) is a birnavirus of economic importance to the poultry industry. The virus infects B cells, causing morbidity, mortality, and immunosuppression in infected birds. In this study, we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with IBDV, and the quantification of viral replication. The addition of chicken CD40 ligand significantly increased cell proliferation fourfold over six days of culture and significantly enhanced cell viability. Two strains of IBDV, a cell-culture adapted strain, D78, and a very virulent strain, UK661, replicated well in the ex vivo cell cultures. This model will be of use in determining how cells respond to IBDV infection and will permit a reduction in the number of infected birds used in IBDV pathogenesis studies. The model can also be expanded to include other viruses and could be applied to different species of birds.

引用

页数：6

共 20 条

[1] Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV) [J].