Adsorption of Glucose Oxidase to 3-D Scaffolds of Carbon Nanotubes: Analytical Applications

被引：38
作者：
Nejadnik, M. Reza [1 ]
Deepak, Francis L. [2 ,3 ]
Garcia, Carlos D. [1 ]
机构：
[1] Univ Texas San Antonio, Dept Chem, San Antonio, TX 78249 USA
[2] Univ Texas San Antonio, Dept Phys & Astron, San Antonio, TX 78249 USA
[3] Int Iberian Nanotechnol Lab, P-4715310 Braga, Portugal
来源：
ELECTROANALYSIS | 2011年 / 23卷 / 06期
基金：
美国国家卫生研究院;
关键词：
Carbon nanotube; Nanostructures; Enzyme adsorption; Ellipsometry; Glucose oxidase; Biosensors; DIRECT ELECTRON-TRANSFER; DIRECT ELECTROCHEMISTRY; OPTICAL-PROPERTIES; SERUM-ALBUMIN; CATALASE; PROTEIN; ENZYME; FILMS; FUNCTIONALIZATION; IMMOBILIZATION;
D O I：
10.1002/elan.201000758
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This study is the first to focus on the potential use of carbon nanotube (CNT) scaffolds as enzyme immobilization substrates for analytical purposes. Besides all the well-known advantages of CNT, three-dimensional scaffolds can significantly increase the amount of enzymes adsorbed per unit area, preserve the catalytic activity of the adsorbed molecules, and allow effective exposure to substrates present in the adjacent medium. Additionally, our results indicate that the sensitivity of analytical probes based on enzyme-loaded CNT scaffolds is proportional to the thickness of the scaffold providing 3-fold sensitivity improvements with respect to the control surfaces.
引用
收藏
页码：1462 / 1469
页数：8
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