The Carbohydrate Metabolism of Lactiplantibacillus plantarum

被引：64
作者：
Cui, Yanhua [1 ]
Wang, Meihong [1 ]
Zheng, Yankun [1 ]
Miao, Kai [1 ]
Qu, Xiaojun [2 ]
机构：
[1] Harbin Inst Technol, Sch Med & Hlth, Dept Food Nutr & Hlth, Harbin 150001, Peoples R China
[2] Heilongjiang Acad Sci, Inst Microbiol, Harbin 150010, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 24期
关键词：
Lactiplantibacillus plantarum; carbohydrate metabolism; two-component system; COMPLETE GENOME SEQUENCE; 2-COMPONENT REGULATORY SYSTEM; LACTOBACILLUS-PLANTARUM; STRAIN; DIVERSITY; RESISTANCE; RESOURCE;
D O I：
10.3390/ijms222413452
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lactiplantibacillus plantarum has a strong carbohydrate utilization ability. This characteristic plays an important role in its gastrointestinal tract colonization and probiotic effects. L. plantarum LP-F1 presents a high carbohydrate utilization capacity. The genome analysis of 165 L. plantarum strains indicated the species has a plenty of carbohydrate metabolism genes, presenting a strain specificity. Furthermore, two-component systems (TCSs) analysis revealed that the species has more TCSs than other lactic acid bacteria, and the distribution of TCS also shows the strain specificity. In order to clarify the sugar metabolism mechanism under different carbohydrate fermentation conditions, the expressions of 27 carbohydrate metabolism genes, catabolite control protein A (CcpA) gene ccpA, and TCSs genes were analyzed by quantitative real-time PCR technology. The correlation analysis between the expressions of regulatory genes and sugar metabolism genes showed that some regulatory genes were correlated with most of the sugar metabolism genes, suggesting that some TCSs might be involved in the regulation of sugar metabolism.
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页数：22
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共 49 条
  • [21] Complete genome sequencing of exopolysaccharide-producing Lactobacillus plantarum K25 provides genetic evidence for the probiotic functionality and cold endurance capacity of the strain[J]. Jiang, Yunyun;Zhang, Jian;Zhao, Xiao;Zhao, Wen;Yu, Zhijian;Chen, Chao;Yang, Zhennai. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2018(07)
  • [22] Complete genome sequence of Lactobacillus plantarum WCFS1[J]. Kleerebezem, M;Boekhorst, J;van Kranenburg, R;Molenaar, D;Kuipers, OP;Leer, R;Tarchini, R;Peters, SA;Sandbrink, HM;Fiers, MWEJ;Stiekema, W;Lankhorst, RMK;Bron, PA;Hoffer, SM;Groot, MNN;Kerkhoven, R;de Vries, M;Ursing, B;de Vos, WM;Siezen, RJ. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003(04)
  • [23] Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation[J]. Koren, Sergey;Walenz, Brian P.;Berlin, Konstantin;Miller, Jason R.;Bergman, Nicholas H.;Phillippy, Adam M. GENOME RESEARCH, 2017(05)
  • [24] Complete Genome Sequence of the Probiotic Lactobacillus plantarum Strain ZJ316[J]. Li, Xuan;Gu, Qing;Lou, Xiuyu;Zhang, Xiaomei;Song, Dafeng;Shen, Lei;Zhao, Yizhen. GENOME ANNOUNCEMENTS, 2013(02)
  • [25] Complete genome sequences and comparative genome analysis of Lactobacillus plantarum strain 5-2 isolated from fermented soybean[J]. Liu, Chen-Jian;Wang, Rui;Gong, Fu-Ming;Liu, Xiao-Feng;Zheng, Hua-Jun;Luo, Yi-Yong;Li, Xiao-Ran. GENOMICS, 2015(06)
  • [26] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Livak, KJ;Schmittgen, TD. METHODS, 2001(04)
  • [27] The carbohydrate-active enzymes database (CAZy) in 2013[J]. Lombard, Vincent;Ramulu, Hemalatha Golaconda;Drula, Elodie;Coutinho, Pedro M.;Henrissat, Bernard. NUCLEIC ACIDS RESEARCH, 2014(D1)
  • [28] Comparative Genomic Analysis of Lactiplantibacillus plantarum Isolated from Different Niches[J]. Mao, Bingyong;Yin, Ruimin;Li, Xiaoshu;Cui, Shumao;Zhang, Hao;Zhao, Jianxin;Chen, Wei. GENES, 2021(02)
  • [29] Nomadic lifestyle of Lactobacillus plantarum revealed by comparative genomics of 54 strains isolated from different habitats[J]. Martino, Maria Elena;Bayjanov, Jumamurat R.;Caffrey, Brian E.;Wels, Michiel;Joncour, Pauline;Hughes, Sandrine;Gillet, Benjamin;Kleerebezem, Michiel;van Hijum, Sacha A. F. T.;Leulier, Francois. ENVIRONMENTAL MICROBIOLOGY, 2016(12)
  • [30] Miao K., 2020, THESIS HARBIN I TECH
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