Hancornia speciosa Gomes (Apocynaceae) as a potential anti-diabetic drug

Ethnopharmacological relevance: The leaves of Hancornia speciosa Gomes are traditionally used to treat diabetes in Brazil. The aim of the study is to evaluate the potential anti-diabetic effect of Hancornia speciosa extract and derived fractions. Materials and Methods: The ethanolic extract from Hancornia speciosa leaves and chromatographic fractions thereof were evaluated on alpha-glucosidase assay, on hyperglycemic effect and glucose uptake. The chemical composition of the extract and its most active fraction was investigated by ESI-LC-MS. Results: The ethanolic extract and derived fractions inhibited alpha-glucosidase in vitro. However, only the crude extract and the dichloromethane fraction inhibited the hyperglycemic effect induced by starch or glucose. Both the extract and dichloromethane fraction were also able to increase glucose uptake in adipocytes. Bornesitol, quinic acid, and chorogenic acid were identified in the extract, along with flavonoid glycosides, whereas the dichloromethane fraction is majorly composed by esters of lupeol and/or alpha/beta-amirin. Conclusions: Hancornia speciosa has a potential anti-diabetic effect through a mechanism dependent on inhibition of alpha-glucosidase and increase on glucose uptake. These results give support to the use on traditional medicine of this medicinal plant. (C) 2014 Elsevier Ireland Ltd. All rights reserved.