Bioprospection of Cytotoxic Compounds in Fungal Strains Recovered from Sediments of the Brazilian Coast

被引：24
|
作者：
Rodrigues, Barbara S. F. [1 ]
Sahm, Bianca D. B. [2 ,3 ]
Jimenez, Paula C. [2 ,3 ]
Pinto, Francisco C. L. [1 ]
Mafezoli, Jair [1 ]
Mattos, Marcos C. [1 ]
Rodrigues-Filho, Edson [4 ]
Pfenning, Ludwig H. [5 ]
Abreu, Lucas M. [5 ]
Costa-Lotufo, Leticia V. [2 ,3 ]
Oliveira, Maria C. F. [1 ]
机构：
[1] Univ Fed Ceara, Dept Quim Organ & Inorgan, BR-60455970 Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, LABOMAR, Inst Ciencias Mar, BR-60185061 Fortaleza, Ceara, Brazil
[3] Univ Fed Ceara, Dept Fisiol & Farmacol, Fortaleza, Ceara, Brazil
[4] Univ Fed Sao Carlos, Dept Quim, BR-13560 Sao Carlos, SP, Brazil
[5] Univ Fed Lavras, Dept Fitopatol, Lavras, MG, Brazil
来源：
CHEMISTRY & BIODIVERSITY | 2015年 / 12卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
Dichotomomyces cejpii; Fungi; Cytotoxic activity; Gliotoxin; Gliotoxin G; acetyl-; Fiscalin B; ANTIVIRAL ACTIVITY; GLIOTOXIN; GLIOCLADIUM; BIOSYNTHESIS; METABOLITES; INHIBITION; DIVERSITY; SUBSTANCE; GROWTH;
D O I：
10.1002/cbdv.201400193
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cytotoxic activities of extracts (50g/ml) from 48 fungal strains, recovered from sediments of Pecem's offshore port terminal (Northeast coast of Brazil), against HCT-116 colon cancer cell lines were investigated. The most promising extract was obtained from strain BRF082, identified as Dichotomomyces cejpii by phylogenetic analyses of partial RPB2 gene sequence. Thus, it was selected for bioassay-guided isolation of the cytotoxic compounds. Large-scale fermentation of BRF082 in potato dextrose broth, followed by chromatographic purification of the bioactive fractions from the liquid medium, yielded gliotoxin (4) and its derivatives acetylgliotoxin G (3), bis(dethio)bis(methylsulfanyl)gliotoxin (1), acetylgliotoxin (5), 6-acetylbis(dethio)bis(methylsulfanyl)gliotoxin (2), besides the quinazolinone alkaloid fiscalin B. All isolated compounds were tested for their cytotoxicities against the tumor cell lines HCT-116, revealing 4 and 3 as the most cytotoxic ones (IC50 0.41 and 1.06g/ml, resp.).
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收藏
页码：432 / 442
页数：11
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