Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp PCC 6803

被引：20

作者：

Zhang, Lifang ^{[1
]}

Selao, Tiago Toscano ^{[1
]}

Selstam, Eva ^{[2
]}

Norling, Birgitta ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Biol Sci, Singapore 639798, Singapore

[2] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, Umea, Sweden

来源：

PLOS ONE | 2015年 / 10卷 / 06期

关键词：

ENERGY-DISSIPATION; LYCOPENE CYCLASE; PLASMA-MEMBRANE; PHOTOSYSTEM-II; SP PCC-6803; CYANOBACTERIUM; PROTEIN; MYXOXANTHOPHYLL; PATHWAY; COMPLEX;

D O I：

10.1371/journal.pone.0130904

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of beta-carotene precursor molecules and echinenone.

引用

页数：13

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