The electrochemistry behaviour of adsorption of BESOD on mercury electrode surface

被引：0

作者：

Qian, W ^{[1
]}

Meng, QG ^{[1
]}

Luo, QH ^{[1
]}

Shen, MC ^{[1
]}

机构：

[1] NANJING UNIV,COORDINAT CHEM STATE KEY LAB,INST COORDINAT CHEM,NANJING 210093,PEOPLES R CHINA

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 1996年 / 17卷 / 05期

关键词：

Cu; Zn-superoxide dismutase; adsorption; cyclic voltammetry; double potential-step chronocoulometry;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The electrochemical behaviour of bovine erythrocyte superoxide dismutase (BESOD) adsorbed on the mercury electrode surface was reported for the first time by cyclic voltammetry, direct current polarography and double potential-step chronoculoumetry without using mediator, The contribution of diffusion current predominated over adsorption current when the concentration of BESOD was more than 1.0x10(-4) mol/L, The experimental adsorption isothermal line was in accordance with that calculated by Langmuir equation, The equilibrium constant of adsorptin was 8.96x10(4) and Gibbs free energy was -28.2 kJ/mol, The molecular area (18.06 nm(2)) of BESOD obtained by the electrochemical method, is in good agreement with that (17.36 nm(2)) determined by X-ray crystal structural analysis.

引用

页码：687 / 689

页数：3

共 10 条

[1] A STUDY OF ADSORPTION OF CADMIUM(2) ON MERCURY FROM THIOCYANATE SOLUTIONS BY DOUBLE POTENTIAL-STEP CHRONOCOULOMETRY
ANSON, FC
CHRISTIE, JH
OSTERYOU.RA
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1967, 13 (04): : 343 - &
[2] INNOVATIONS IN STUDY OF ADSORBED REACTANTS BY CHRONOCOULOMETRY
ANSON, FC
[J]. ANALYTICAL CHEMISTRY, 1966, 38 (01) : 54 - &
[3] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS
ARMSTRONG, FA
HILL, HAO
WALTON, NJ
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1988, 21 (11) : 407 - 413
[4] REDOX CHEMISTRY OF SUPEROXIDE-DISMUTASE - CYCLIC VOLTAMMETRY OF WILD-TYPE ENZYMES AND MUTANTS ON FUNCTIONALLY RELEVANT RESIDUES
AZAB, HA
BANCI, L
BORSARI, M
LUCHINAT, C
SOLA, M
VIEZZOLI, MS
[J]. INORGANIC CHEMISTRY, 1992, 31 (22) : 4649 - 4655
[5] Clair C.S. Ct, 1992, INORG CHEM, V31, P925
[6] HALLIWELL B, 1976, FEBS LETT, V72, P8, DOI 10.1016/0014-5793(76)80801-0
[7] MCCORD JM, 1969, J BIOL CHEM, V244, P6049
[8] RICHARDSON DC, 1972, J BIOL CHEM, V247, P6368
[9] ROLE OF IRON AND ETHYLENEDIAMINETETRAACETIC ACID IN THE BACTERICIDAL ACTIVITY OF A SUPEROXIDE ANION-GENERATING SYSTEM
ROSEN, H
KLEBANOFF, SJ
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 208 (02) : 512 - 519
[10] EFFECTS OF ADSORPTION OF ELECTROACTIVE SPECIES IN STATIONARY ELECTRODE POLAROGRAPHY
WOPSCHALL, RH
SHAIN, I
[J]. ANALYTICAL CHEMISTRY, 1967, 39 (13) : 1514 - +

← 1 →