A novel photoswitchable enzyme cascade for powerful signal amplification in versatile bioassays

被引：25

作者：

Wang, Guang-Li ^{[1
]}

Li, Xiao-Qin ^{[1
]}

Cao, Gen-Xia ^{[1
]}

Yuan, Fang ^{[1
]}

Dong, Yuming ^{[1
]}

Li, Zaijun ^{[1
]}

机构：

[1] Jiangnan Univ, Key Lab Synthet Colloids & Biotechnol, Minist Educ, Sch Chem & Mat Engn, Wuxi 214122, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 81期

基金：

中国国家自然科学基金;

关键词：

TYROSINASE ACTIVITY; MAGNETIC NANOPARTICLES; GOLD NANOCLUSTERS; QUANTUM DOTS; SURFACE; ELECTRODES; COMPLEXES; DOPAMINE; PROTEIN; TIO2;

D O I：

10.1039/c7cc05771g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This report outlines the construction of an advanced, exquisite photoswitchable enzyme cascade on the basis that tyrosinase (TYR) catalyzes the generation of dihydroxyphenylalanine ( DOPA) coordinated TiO2 nanoparticles (NPs) to form a light responsive nano-trigger that subsequently photoactivates the enzymatic activity of horseradish peroxidase (HRP). This photoswitchable enzyme cascade has a powerful signal transduction/amplification ability in TYR-based bioassays, and holds great promise to be applied in versatile applications.

引用

页码：11165 / 11168

页数：4

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