Putative telomere-independent mechanisms of replicative aging reflect inadequate growth conditions

被引：365

作者：

Ramirez, RD ^{[1
]}

Morales, CP ^{[1
]}

Herbert, BS ^{[1
]}

Rohde, JM ^{[1
]}

Passons, C ^{[1
]}

Shay, JW ^{[1
]}

Wright, WE ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Cell Biol, Dallas, TX 75390 USA

来源：

GENES & DEVELOPMENT | 2001年 / 15卷 / 04期

关键词：

replicative aging; cellular senescence; telomeres; p16(INK4a); aging; cancer;

D O I：

10.1101/gad.859201

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Telomere shortening is the mechanism underlying replicative aging in fibroblasts. A variety of reports now claim that inactivation of the p16(INK4a)/pRB pathway is required in addition to telomere maintenance for the immortalization of cells such as skin keratinocytes and breast epithelial cells. We here show that the premature growth arrest of these cell types can be explained by an inadequate culture environment. Providing mesenchymal/epithelial interactions by cultivating the telomerase-expressing cells on feeder layers avoids the growth arrest associated with increased p16(INK4a). These results do not support a telomere-independent mechanism of replicative aging.

引用

页码：398 / 403

页数：6

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