Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice

被引：68

作者：

Kathe, Claudia ^{[1
,2
,3
,4
]}

Michoud, Frederic ^{[5
]}

Schoenle, Philipp ^{[6
]}

Rowald, Andreas ^{[1
,2
,3
,4
]}

Brun, Noe ^{[6
]}

Ravier, Jimmy ^{[1
,2
,3
,4
]}

Furfaro, Ivan ^{[5
]}

Paggi, Valentina ^{[5
]}

Kim, Kyungjin ^{[5
]}

Soloukey, Sadaf ^{[7
,8
]}

Asboth, Leonie ^{[1
,2
,3
,4
]}

Hutson, Thomas H. ^{[1
,2
,3
,4
]}

Jelescu, Ileana ^{[9
]}

Philippides, Antoine ^{[10
]}

Alwahab, Noaf ^{[5
]}

Gandar, Jerome ^{[1
,2
,3
,4
]}

Huber, Daniel ^{[10
]}

De Zeeuw, Chris, I ^{[7
,11
]}

Barraud, Quentin ^{[1
,2
,3
,4
]}

Huang, Qiuting ^{[6
]}

Lacour, Stephanie P. ^{[5
]}

Courtine, Gregoire ^{[1
,2
,3
,4
,12
]}

机构：

[1] Swiss Fed Inst Technol EPFL, Sch Life Sci, Ctr Neuroprosthet, Geneva, Switzerland

[2] Swiss Fed Inst Technol EPFL, Sch Life Sci, Brain Mind Inst, Geneva, Switzerland

[3] Univ Lausanne, Univ Hosp Lausanne CHUV, Defitech Ctr Intervent Neurotherapies NeuroRestor, Lausanne, Switzerland

[4] Ecole Polytech Fed Lausanne, Lausanne, Switzerland

[5] EPFL, Bertarelli Fdn Chair Neuroprosthet Technol, Ctr Neuroprosthet, Inst Bioengn,Inst Microenginnering,Lab Soft Bioel, Geneva, Switzerland

[6] Swiss Inst Technol Zurich, Dept Informat Technol & Elect Engn, Integrated Syst Lab, Zurich, Switzerland

[7] Erasmus MC, Dept Neurosci, Rotterdam, Netherlands

[8] Erasmus MC, Dept Neurosurg, Rotterdam, Netherlands

[9] Ecole Polytech Fed Lausanne, Ctr Imagerie Biomed, Lausanne, Switzerland

[10] Univ Geneva, Dept Fundamental Neurosci, Geneva, Switzerland

[11] Royal Dutch Acad Arts & Sci, Netherlands Inst Neurosci, Amsterdam, Netherlands

[12] CHU Vaudois, Dept Neurosurg, Lausanne, Switzerland

来源：

NATURE BIOTECHNOLOGY | 2022年 / 40卷 / 02期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

CIRCUIT REORGANIZATION; LOCOMOTOR RECOVERY; OPTOELECTRONICS; BRAIN;

D O I：

10.1038/s41587-021-01019-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone-phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photo-stimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice.

引用

页码：198 / +

页数：35

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