Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500-1800 cm-1

被引：7

作者：

Hakert, Hubertus ^{[1
]}

Eibl, Matthias ^{[2
]}

Tillich, Marie ^{[1
]}

Pries, Ralph ^{[3
]}

Huettmann, Gereon ^{[2
]}

Brinkmann, Ralf ^{[1
]}

Wollenberg, Barbara ^{[3
,4
]}

Bruchhage, Karl-Ludwig ^{[3
]}

Karpf, Sebastian ^{[2
]}

Huber, Robert ^{[2
]}

机构：

[1] Med Lasetzentrum Lubeck GmbH, Peter Monnik Weg 4, D-23562 Lubeck, Germany

[2] Univ Lubeck, Inst Biomed Opt, Peter Monnik Weg 4, D-23562 Lubeck, Germany

[3] Univ Klinikum Schleswig Holstein Lubeck, Klin Hals Nasen & Ohrenheilkunde, Ratzeburger Allee 160, D-23562 Lubeck, Germany

[4] Klinikum Rechts Der Isar, Klin & Poliklin Hals Nasen & Ohrenheilkunde, Ismaninger Str 22, D-81675 Munich, Germany

来源：

OPTICS LETTERS | 2021年 / 46卷 / 14期

基金：

欧洲研究理事会;

关键词：

IN-VIVO DIAGNOSIS; LIGHT-SOURCES; FIBER LASER; SPECTROSCOPY;

D O I：

10.1364/OL.424726

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500 1800 cm(-1). Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications. (C) 2021 Optical Society of America

引用

页码：3456 / 3459

页数：4

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