LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization

被引：38
作者：
Li, Xueqin [1 ,2 ]
Zhang, Yingying [1 ,3 ]
Pei, Weiya [1 ,2 ]
Zhang, Mengying [1 ,2 ]
Yang, Hui [1 ,2 ]
Zhong, Min [1 ,2 ]
Kong, Xiang [1 ,2 ]
Xu, Yang [1 ,2 ]
Zhu, Xiaolong [1 ,2 ]
Chen, Tianbing [1 ,2 ]
Ye, Jingjing [1 ,2 ]
Lv, Kun [1 ,2 ]
机构：
[1] Wannan Med Coll, Key Lab Noncoding RNA Transformat Res, Anhui Higher Educ Inst, Wuhu, Peoples R China
[2] Wannan Med Coll, Cent Lab, Yijishan Hosp, 2 Western Zheshan Rd, Wuhu 241001, Peoples R China
[3] Wannan Med Coll, Yijishan Hosp, Lab Med, Wuhu, Peoples R China
来源：
FASEB JOURNAL | 2020年 / 34卷 / 04期
基金：
中国国家自然科学基金;
关键词：
DNA methylation; Dnmt3a; LncRNA; macrophage polarization; LONG NONCODING RNA; GENE-EXPRESSION; DIFFERENTIATION; TRANSCRIPTION; MICROARRAY; INFLAMMATION; ACTIVATION; INTERACTS; PROFILES; PATTERNS;
D O I：
10.1096/fj.201902379R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Long non-coding RNAs (lncRNAs) play key roles in various biological processes. However, the roles of lncRNAs in macrophage polarization remain largely unexplored. In this study, thousands of lncRNAs were identified that are differentially expressed in distinct polarized bone marrow-derived macrophages. Among them, Dnmt3aos (DNA methyltransferase 3A, opposite strand), as a known lncRNA, locates on the antisense strand of Dnmt3a. Functional experiments further confirmed that Dnmt3aos were highly expressed in M(IL-4) macrophages and participated in the regulation of Dnmt3a expression, and played a key role in macrophage polarization. The DNA methylation profiles between the Dnmt3aos knockdown group and the control group in M(IL-4) macrophages were determined by MeDIP-seq technique for the first time, and the Dnmt3aos-Dnmt3a axis-mediated DNA methylation modification-regulated macrophage polarization- related gene IFN-gamma was identified. Our study will help to enrich our knowledge of the mechanism of macrophage polarization.
引用
收藏
页码：5077 / 5091
页数：15
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