Purification and partial characterization of two chitinases from the mycoparasitic fungus Talaromyces flavus

被引：35

作者：

Duo-Chuan, LI ^{[1
]}

Chen, S ^{[1
]}

Jing, LU ^{[1
]}

机构：

[1] Shandong Agr Univ, Dept Plant Pathol, Shandong 271018, Peoples R China

来源：

MYCOPATHOLOGIA | 2005年 / 159卷 / 02期

基金：

中国国家自然科学基金;

关键词：

chitinase; Talaromyces flavus;

D O I：

10.1007/s11046-004-9096-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chitinases were produced by Talaromyces flavus CGMCC 3.4301 when it was grown in the presence of chitin. Two chitinases from the culture filtrate of T. flavus were purified to homogeneity by fractional ammonium sulphate precipitation, ion-exchange chromatography on DEAE-Sepharose and Phenyl Sepharose hydrophobic interaction chromatography. By SDS-PAGE, the molecular weight of the two enzymes was estimated to be 41 and 32 kDa, respectively. The 41 kDa chitinase (CHIT41) had a 4.0 pH optimum; the 32 kDa chitinase (CHIT32) optimum activity was at pH 5.0. The optimum temperature for the two chitinase activities was 40 degrees C. The two chitinases had activity against cell wall of Verticillium dahliae, Sclerotinia sclerotiorum and Rhizoctonia solani, and inhibited spore germination and germ tube elongation of Alternaria alternata, Fusarium moniliforme, and Magnaporthe grisea.

引用

页码：223 / 229

页数：7

共 27 条

[1] CHITINASE IN BEAN-LEAVES - INDUCTION BY ETHYLENE, PURIFICATION, PROPERTIES, AND POSSIBLE FUNCTION [J].

BOLLER, T ;

GEHRI, A ;

MAUCH, F ;

VOGELI, U .

PLANTA, 1983, 157 (01) :22-31

[2]

BOOSALIS MG, 1956, PHYTOPATHOLOGY, V46, P473

[3]

DAVIS JR, 1986, BIOL CULT TESTS CONT, V1, P18

[4]

DELACRUZ J, 1992, EUR J BIOCHEM, V206, P859, DOI 10.1111/j.1432-1033.1992.tb16994.x

[5]

DIPIETRO A, 1993, PHYTOPATHOLOGY, V83, P308, DOI 10.1094/Phyto-83-308

[6]

DUTTA BK, 1981, PLANT SOIL, V63, P209, DOI 10.1007/BF02374599

[7] QUANTITATIVE ASSESSMENT OF THE INTERACTION BETWEEN THE ANTAGONISTIC FUNGUS TALAROMYCES-FLAVUS AND THE WILT PATHOGEN VERTICILLIUM-DAHLIAE ON EGGPLANT ROOTS [J].

FAHIMA, T ;

HENIS, Y .

PLANT AND SOIL, 1995, 176 (01) :129-137

[8]

Fahima T., 1992, Biocontrol Science and Technology, V2, P69, DOI 10.1080/09583159209355220

[9]

FAHIMA T, 1990, BIOLOGICAL CONTROL OF SOIL-BORNE PLANT PATHOGENS, P165

[10]

FRAVEL DR, 1986, BIOL CULT TESTS, V1, P7

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