Molecular detection of Toxoplasma gondii in feathered game intended for human consumption in the Czech Republic

被引：11
作者：
Skorpikova, Lucie [1 ,2 ]
Reslova, Nikol [1 ,2 ]
Lorencova, Alena [1 ]
Plhal, Radim [3 ]
Drimaj, Jakub [3 ]
Kamler, Jiri [3 ]
Slany, Michal [1 ]
机构：
[1] Vet Res Inst, Dept Food & Feed Safety, Hudcova 70, Brno 62100, Czech Republic
[2] Masaryk Univ, Fac Sci, Dept Bot & Zool, Kamenice 5, Brno 62500, Czech Republic
[3] Mendel Univ Brno, Dept Forest Protect & Wildlife Management, Fac Forestry & Wood Technol, Zemedelska 3, Brno 61300, Czech Republic
来源：
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2018年 / 286卷
关键词：
Toxoplasmosis; Food safety; Real-time PCR; Genotyping; Ducks; Pheasants; WILD BIRDS; REPEAT ELEMENT; 1ST DETECTION; PCR; PREVALENCE; INFECTION; IDENTIFICATION; WATERFOWL; AF146527; PARASITE;
D O I：
10.1016/j.ijfoodmicro.2018.07.019
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Toxoplasma gondii is an important ubiquitous protozoan parasite, which can infect almost all warm-blooded vertebrates, including humans. The diagnosis of T. gondii infection is crucial for the prevention, surveillance, and control of its transmission. Here, a triplex real-time PCR assay targeting the B1 gene and 529rep element was used to determine the presence of T. gondii in feathered game (Anas platyrhynchos and Phasianus colchicus) hunted in the Czech Republic. The prevalence of T. gondii was 5.4% in wild ducks (n = 280) and 3.4% in common pheasants (n = 350). Additionally, genotyping of 28 T. gondii-positive samples revealed the presence of archetypal genotypes II and III as well as non-archetypal genotypes combining both type II and III alleles. Our results suggest that consumption of feathered game could pose a risk of T. gondit transmission to humans in the Czech Republic.
引用
收藏
页码：75 / 79
页数：5
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