The effect of concentration and duration of normobaric oxygen in reducing caspase-3 and-9 expression in a rat-model of focal cerebral ischaemia

被引:12
作者
Chen, Suyan [1 ]
Peng, Huizhen [1 ]
Rowat, Anne [2 ]
Gao, Feng [3 ]
Zhang, Zhenxiang [1 ]
Wang, Peng [1 ]
Zhang, Weihong [1 ]
Wang, Xianyuan [4 ]
Qu, Lixia [1 ]
机构
[1] Zhengzhou Univ, Dept Med Nursing, Sch Nursing, Zhengzhou 450052, Henan, Peoples R China
[2] Edinburgh Napier Univ, Sch Nursing Midwifery & Social Care, Edinburgh, Midlothian, Scotland
[3] Zhengzhou Univ, Dept Neuroimmunol, Henan Acad Med & Pharmaceut Sci, Zhengzhou 450052, Henan, Peoples R China
[4] Zhengzhou Univ, Affiliated Hosp 2, Dept Pathol, Zhengzhou 450052, Henan, Peoples R China
关键词
Normobaric oxygen; Ischaemic rat model; Cerebral ischaemic-reperfusion; Apoptosis; ARTERY OCCLUSION; INFARCT VOLUME; STROKE; THERAPY; APOPTOSIS; DELAYS;
D O I
10.1016/j.brainres.2015.05.027
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The aim of this study was to determine the effect of different concentrations of normobaric oxygen (NBO) on neurological function and the expression of caspase-3 and -9 in a rat model of acute cerebral ischaemia. Sprague-Dawley rats (n=120) were randomly divided into four groups (n=30 per group), including 3 groups given NBO at concentrations of 33%, 45% or 61% and one control group given air (21% oxygen). After 2 h of ischaemic occlusion, each group was further subdivided into six subgroups (n=5) during reperfusion according to the duration (3, 6, 12, 24, 48 or 72 h) and concentration of NBO (33%, 45% or 61%) or air treatment. The Fluorescence Quantitative polymerase chain reaction (PCR) and immunohistochemistry were used to detect caspase-3 and -9 mRNA and protein relative expression respectively. The Neurologic Impairment Score (NIS) was significantly lower in rats given 61% NBO >= 3 h after reperfusion when compared to the control group (P<0.05, Mann-Whitney U). NBO significantly reduced caspase-3 and -9 mRNA and protein expression when compared to the control group at all NBO concentrations and time points (P<0.05, ANOVA). The expression of caspase-3 and -9 was lower in the group given 61% NBO compared any other group, and this difference was statistically significant when compared to the group given 33% NBO for >= 48 h and the control group (both P<0.05, ANOVA). These findings indicate that NBO may inhibit the apoptotic pathway by reducing caspase-3 and -9 expression, thereby promoting neurological functional recovery after stroke. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:205 / 211
页数:7
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