Hyaluronic acid-polyethyleneimine conjugate for target specific intracellular delivery of siRNA

被引：127

作者：

Jiang, Ge ^{[1
,2
,3
]}

Park, Kitae ^{[4
]}

Kim, Jiseok ^{[1
]}

Kim, Ki Su ^{[1
]}

Oh, Eun Ju ^{[1
]}

Kang, Hyungu

Han, Su-Eun ^{[5
]}

Oh, Yu-Kyoung ^{[5
]}

Park, Tae Gwan ^{[6
]}

Hahn, Sei Kwang ^{[1
,4
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Mat Sci & Engn, Pohang 790784, South Korea

[2] Dalian Univ Technol, Bioengn Coll, Dalian 116622, Liaoning, Peoples R China

[3] Dalian Univ Technol, Key Lab Bioorgan Chem, Dalian 116622, Liaoning, Peoples R China

[4] POSTECH I Bio Program, Pohang 790784, South Korea

[5] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[6] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

来源：

BIOPOLYMERS | 2008年 / 89卷 / 07期

关键词：

hyaluronic acid; polyethyleneimine; small interfering RNA; LYVE-1 HA receptor; intracellular gene delivery;

D O I：

10.1002/bip.20978

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel target specific small interfering RNA (siRNA) delivery system was successfully developed using I polyethyleneimine (PEI)-hyaluronic acid (HA) conjugate. Anti-PGL3-Luc siRNA was used as a model system suppressing the PGL3-Luc gene expression. The siRNA/PEI-HA complex with an average size of ca.21 nm appeared to be formed by electrostatic interaction between the negatively charged siRNA and the positively charged PEI of PEI-HA conjugate. The cytotoxicity of siRNA/PEI-HA complex to B16F1 cells was lower than that of siRNA/PEI complex according to the MTTassay. When B16F1 and HEK-293 cells were treated with fluorescein isothiocyanate (FITC) labeled siRNA/PEI-HA complex, B16F1 cells, with a lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), showed higher green fluorescent intensity than HEK-293 cells because of the HA receptor mediated endocytosis of the complex. Accordingly, the PGL3-Luc gene silencing of anti-PGL3-Luc siRNA/PEI-HA complex was more efficient in B16F1 cells than in HEK-293 cells. In addition, the inhibited PGL3-Luc gene silencing effect in the presence of free HA in the transfection medium revealed that siRNA/HA-PEI complex was selectively taken up to B16F1 cells via HA receptor mediated endocytosis. All these results demonstrated that the intracellular delivery of anti-PGL3-Luc siRNA/PEI-HA complex could be facilitated by the HA receptor mediated endocytosis. (c) 2008 Wiley Periodicals, Inc.

引用

页码：635 / 642

页数：8

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