Byakangelicin inhibits IL-1 β-induced mouse chondrocyte inflammation in vitro and ameliorates murine osteoarthritis in vivo

被引:13
作者
Zhang, Tan [1 ]
He, Lei [1 ]
Yang, Wanlei [1 ]
Wang, Yanben [1 ]
Peng, Jiaxuan [4 ]
Sun, Peng [2 ,3 ]
Yang, Qichang [2 ,3 ]
Jia, Yewei [1 ]
Zhao, Kanxian [2 ,3 ]
Qian, Yu [1 ]
机构
[1] Zhejiang Univ, Shaoxing Hosp, Dept Orthoped, Sch Med, Shaoxing 312000, Zhejiang, Peoples R China
[2] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Zhejiang, Peoples R China
[3] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China
[4] Guangxi Med Univ, Guangxi Key Lab Regenerat Med, Guangxi 530021, Peoples R China
基金
中国国家自然科学基金;
关键词
Byakangelicin; Chondrocyte; NF-kappa B; IL-1; beta; Inflammation; Osteoarthritis; NF-KAPPA-B; TNF-ALPHA; EXPRESSION; CARTILAGE; TARGET; COX-2;
D O I
10.1016/j.intimp.2020.106605
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Osteoarthritis (OA) is a chronic musculoskeletal degeneration disease, resulting in severe consequences such as chronic pain and functional disability. Owing to the complex pathology, there are currently available preventative clinical therapies for OA. Several studies have reported the potential anti-inflammatory effects of byakangelicin (BYA), a component of the Angelica dahurica root extract; however, the effects of BYA in OA remain unknown. In this study, we investigated the protective effects of BYA in interleukin (IL)-1 beta-induced mouse chondrocytes in vitro and on surgical destabilization in a medial meniscus (DMM) mouse OA model in vivo. In vitro, BYA treatment inhibited IL-1 beta-mediated inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha, and IL-6 expression. Moreover, BYA promoted the expression of type two collagen and aggrecan but inhibited the expression of thrombospondin motifs 5 and matrix metalloproteinases, leading to degradation of the extracellular matrix. In addition, BYA mechanistically suppressed nuclear factor-kappa B signaling in the IL-1 beta-induced chondrocytes. The protective effects of BYA in OA development were also observed in vivo using the DMM model. In conclusion, our results highlight BYA as a candidate for OA treatment and prevention.
引用
收藏
页数:11
相关论文
共 48 条
[1]   Osteoarthritis and nitric oxide [J].
Abramson, Steven B. .
OSTEOARTHRITIS AND CARTILAGE, 2008, 16 :S15-S20
[2]  
[Anonymous], 2005, PHARMACOPOEIA PEOPLE
[3]   Elevation of Serum Tumor Necrosis Factor α in Patients with Periprosthetic Osteolysis: A Case-Control Study [J].
Chaganti, R. Krishna ;
Purdue, Edward ;
Sculco, Thomas P. ;
Mandl, Lisa A. .
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 2014, 472 (02) :584-589
[4]   Critical role for arginase II in osteoarthritis pathogenesis [J].
Choi, Wan-Su ;
Yang, Jeong-In ;
Kim, Wihak ;
Kim, Hyo-Eun ;
Kim, Seul-Ki ;
Won, Yoonkyung ;
Son, Young-Ok ;
Chun, Churl-Hong ;
Chun, Jang-Soo .
ANNALS OF THE RHEUMATIC DISEASES, 2019, 78 (03) :421-428
[5]  
Chowdhury TT, 2006, BIORHEOLOGY, V43, P413
[6]   EPIDEMIOLOGY OF NSAID-RELATED GASTROINTESTINAL SIDE-EFFECTS [J].
GIERCKSKY, KE ;
HUSEBY, G ;
RUGSTAD, HE .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1989, 24 :3-8
[7]  
Graf E, 1992, PHARMAZIE, V21, P281
[8]   Association of Pharmacological Treatments With Long-term Pain Control in Patients With Knee Osteoarthritis A Systematic Review and Meta-analysis [J].
Gregori, Dario ;
Giacovelli, Giampaolo ;
Minto, Clara ;
Barbetta, Beatrice ;
Gualtieri, Francesca ;
Azzolina, Danila ;
Vaghi, Paola ;
Rovati, Lucio C. .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2018, 320 (24) :2564-2579
[9]   Radiocarbon dating reveals minimal collagen turnover in both healthy and osteoarthritic human cartilage [J].
Heinemeier, Katja M. ;
Schjerling, Peter ;
Heinemeier, Jan ;
Moller, Mathias B. ;
Krogsgaard, Michael R. ;
Grum-Schwensen, Tomas ;
Petersen, Michael M. ;
Kjaer, Michael .
SCIENCE TRANSLATIONAL MEDICINE, 2016, 8 (346)
[10]   Shifting HIFs in osteoarthritis [J].
Husa, Matthew ;
Liu-Bryan, Ru ;
Terkeltaub, Robert .
NATURE MEDICINE, 2010, 16 (06) :641-644