RNA-Seq Profiling of Circular RNAs and the Oncogenic Role of circPVT1 in Cutaneous Squamous Cell Carcinoma

被引：16
作者：
Chen, Shuang [1 ]
Ding, Junli [1 ]
Wang, Yunlin [1 ]
Lu, Tao [1 ]
Wang, Lili [1 ]
Gao, Xinghua [1 ]
Chen, Hongduo [1 ]
Qu, Le [1 ]
He, Chundi [1 ]
机构：
[1] China Med Univ, Hosp China Med Univ, Hosp 1, Key Lab Immunohematol,Dept Dermatol, Shenyang, Liaoning, Peoples R China
来源：
ONCOTARGETS AND THERAPY | 2020年 / 13卷
关键词：
circRNA; RNA-seq; CSCC; circPVT1; EXPRESSION; MIR-497; IDENTIFICATION; PROGRESSION; EVOLUTION; DATABASE; ABUNDANT; CIRCRNA;
D O I：
10.2147/OTT.S252233
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background: Cutaneous squamous cell carcinoma (CSCC) is associated with a poor 5-year survival rate. circRNAs have an important role in a number of physiological and pathological processes. However, the relationship between circRNAs and cutaneous squamous cell carcinoma (CSCC) is unclear. Purpose: The aim of the present study was to investigate the expression of circRNAs in cutaneous squamous cell carcinoma (CSCC) and its effect on CSCC proliferation and metastasis. Methods: We used high-throughput sequencing (RNA-seq) to identify circRNAs that were differentially in CSCC tissue and their paracarcinoma tissue. Quantitative real-time PCR results confirm deep-sequencing findings in CSCC tissue and cell lines. CCK-8 assay and flow cytometry were used to detect the effect of circPVT1 on the proliferation and migration of CSCC cells. Results: We identified 449 circRNAs that were differentially expressed between CSCC and normal adjacent tissue samples. circPVT1 (hsa_circ_0001821) was further researched to confirm its oncogene role in CSCC. Conclusion: Differentially expressed circular RNA plays an important role in the development of CSCC, and circPVT1 may be an important target for the treatment of CSCC.
引用
收藏
页码：6777 / 6788
页数：12
相关论文
共 42 条
[1]   Upregulated circular RNA circ_0070934 facilitates cutaneous squamous cell carcinoma cell growth and invasion by sponging miR-1238 and miR-1247-5p [J].
An, Xiaoxia ;
Liu, Xiguang ;
Ma, Guozhang ;
Li, Chen .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 513 (02) :380-385
[2]  
[Anonymous], 2018, J NATL CANC I
[3]   Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer [J].
Chen, Jie ;
Li, Yan ;
Zheng, Qiupeng ;
Bao, Chunyang ;
He, Jian ;
Chen, Bin ;
Lyu, Dongbin ;
Zheng, Biqiang ;
Xu, Yu ;
Long, Ziwen ;
Zhou, Ye ;
Zhu, Huiyan ;
Wang, Yanong ;
He, Xianghuo ;
Shi, Yingqiang ;
Huang, Shenglin .
CANCER LETTERS, 2017, 388 :208-219
[4]   Regulation of circRNA biogenesis [J].
Chen, Ling-Ling ;
Yang, Li .
RNA BIOLOGY, 2015, 12 (04) :381-388
[5]   Specific identification and quantification of circular RNAs from sequencing data [J].
Cheng, Jun ;
Metge, Franziska ;
Dieterich, Christoph .
BIOINFORMATICS, 2016, 32 (07) :1094-1096
[6]  
Cheng Z.-X., 2019, NAT COMMUN, V10, P1
[7]   A circular RNA circ-DNMT1 enhances breast cancer progression by activating autophagy [J].
Du, William W. ;
Yang, Weining ;
Li, Xiangmin ;
Awan, Faryal Mehwish ;
Yang, Zhenguo ;
Fang, Ling ;
Lyu, Juanjuan ;
Li, Feiya ;
Peng, Chun ;
Krylov, Sergey N. ;
Xie, Yizhen ;
Zhang, Yaou ;
He, Chengyan ;
Wu, Nan ;
Zhang, Chao ;
Sdiri, Mouna ;
Dong, Jun ;
Ma, Jian ;
Gao, Chunqi ;
Hibberd, Steven ;
Yang, Burton B. .
ONCOGENE, 2018, 37 (44) :5829-5842
[8]   CircInteractome: A web tool for exploring circular RNAs and their interacting proteins and microRNAs [J].
Dudekulay, Dawood B. ;
Panda, Amaresh C. ;
Grammatikakis, Ioannis ;
De, Supriyo ;
Abdelmohsen, Kotb ;
Gorospe, Myriam .
RNA BIOLOGY, 2016, 13 (01) :34-42
[9]   circBase: a database for circular RNAs [J].
Glazar, Petar ;
Papavasileiou, Panagiotis ;
Rajewsky, Nikolaus .
RNA, 2014, 20 (11) :1666-1670
[10]   Natural RNA circles function as efficient microRNA sponges [J].
Hansen, Thomas B. ;
Jensen, Trine I. ;
Clausen, Bettina H. ;
Bramsen, Jesper B. ;
Finsen, Bente ;
Damgaard, Christian K. ;
Kjems, Jorgen .
NATURE, 2013, 495 (7441) :384-388
12345