Single-step method for the determination of the amount of the common deletion in mitochondrial DNA

被引：7
作者：
Ahmed, F
Kougianos, E
Cummins, JM
Jequier, AM
Whelan, J [1 ]
机构：
[1] Univ Western Australia, Dept Biochem, Nedlands, WA 6907, Australia
[2] Murdoch Univ, Murdoch, WA 6150, Australia
[3] King Edward Mem Hosp, Subiaco, WA, Australia
来源：
BIOTECHNIQUES | 1999年 / 26卷 / 02期
关键词：
D O I：
10.2144/99262st03
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A Florescent-based quantitative PCR assay to measure the percentage of the common deletion in rat mitochondria is reported. The amount of the common deletion is measured relative to the amount of total mitochondrial (mt)DNA. The use of a heterologous competitor construct allows the reaction to be monitored to ensure that exponential accumulation of products occurs. The use of fluorescence-labeled primers provides a safe and sensitive means to quantify products without any adjustment for size. This assay will allow the quantitative determination of the common deletion with one dilution range.
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页码：290 / +
页数：6
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