Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue

被引：10

作者：

Choi, Ji-Woo ^{[1
]}

Hong, Seung Taek ^{[1
]}

Kang, Dong Eun ^{[2
]}

Paik, Kyu Cheol ^{[3
]}

Han, Man So ^{[3
]}

Lim, Chang Su ^{[4
]}

Cho, Bong Rae ^{[1
,2
,3
]}

机构：

[1] Korea Univ, KU KIST Grad Sch Converging Sci & Technol, 145 Anam Ro, Seoul 02841, South Korea

[2] Korea Univ, Dept Chem, 145 Anam Ro, Seoul 02841, South Korea

[3] Daejin Univ, Dept Chem, 1007 Hoguk Ro, Pochun Si 11159, Gyeonggi Do, South Korea

[4] Ajou Univ, Dept Chem, 206 Worldcup Ro, Suwon 16499, Gyeonggi Do, South Korea

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 19期

基金：

新加坡国家研究基金会;

关键词：

IN-SITU HYBRIDIZATION; AFFIBODY MOLECULES; HER-2/NEU; PROTEIN; AMPLIFICATION; MICROSCOPY; HER2; OVEREXPRESSION; CARCINOMAS; EXPRESSION;

D O I：

10.1021/acs.analchem.6b00912

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two photon absorption cross-section value of 40 X 10(-50) cm(4)s/photon (GM) at 750 nm in aqueous buffer solution. The effective two photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 mu m in live mouse tissue by TPM.

引用

页码：9412 / 9418

页数：7

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