Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

被引：0

作者：

Richner, Mette ^{[1
]}

Goncalves, Nadia Pereira ^{[1
]}

Jensen, Poul Henning ^{[3
]}

Nyengaard, Jens Randel ^{[2
]}

Vaegter, Christian Bjerggaard ^{[1
]}

Jan, Asad ^{[3
]}

机构：

[1] Aarhus Univ, Danish Res Inst Translat Neurosci DANDRITE, Nord EMBL Partnership Mol Med, Hoegh Guldbergs Gade 10, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, Core Ctr Mol Morphol, Sect Stereol & Microscopy, Dept Clin Med,Dept Pathol,Aarhus Univ Hosp, DK-8200 Aarhus N, Denmark

[3] Aarhus Univ, Dept Biomed, DANDRITE, Ole Worms Alle 3, DK-8000 Aarhus C, Denmark

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 01期

关键词：

SCIATIC-NERVE; AAV; THERAPY; INJECTION; STRATEGY; VECTOR;

D O I：

10.1016/j.xpro.2022.101181

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol outlines a minimally invasive and quickly performed approach for transgene delivery in the extracranial nervous system of adult mice using recombinant adeno-associated virus (AAV). The technique, named Sciatic Nerve Direct Immersion (SciNDi), relies on the direct bilateral immersion of the exposed sciatic nerve with AAV. We show that in comparison with intramuscular AAV delivery, SciNDi results in widespread transduction in connected neuroanatomical tracts both in the sciatic nerve trunk and the lumbar spinal cord.For complete details on the use and execution of this protocol, please refer to Jan et al. (2019) and Richner et al. (2011, 2017).

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页数：15

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