Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection

被引：35

作者：

Wu, Hongliang ^{[1
,4
]}

Li, Guoliang ^{[2
]}

Liu, Shucheng ^{[3
]}

Liu, Di ^{[1
,4
]}

Chen, Guang ^{[2
]}

Hu, Na ^{[1
,4
]}

Suo, Yourui ^{[1
]}

You, Jinmao ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China

[2] Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China

[3] Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China

[4] Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2015年 / 107卷

基金：

中国国家自然科学基金;

关键词：

Triterpenic acids; Pre-column derivatization; High-performance liquid chromatography; Ultrasound-assisted dispersive liquid-liquid microextraction; Response surface methodology; Traditional Chinese medicinal herbs; SOLID-PHASE EXTRACTION; URSOLIC ACID; OLEANOLIC ACID; LABELING REAGENT; FATTY-ACIDS; HPLC; SERUM; L;

D O I：

10.1016/j.jpba.2014.10.031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) follow by high-performance liquid chromatography (HPLC) with fluorescence detection. Six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. After the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7H-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The limits of detection (LODs) and the limits of quantification (LOQs) were in the range of 0.95-1.36 ng mL(-1) and 3.17-4.55 ng mL(-1), respectively. Under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional Chinese medicinal herbs. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：98 / 107

页数：10

共 31 条

[1]

Arti Gupta Arti Gupta, 2013, Pharmacognosy Journal, V5, P156, DOI 10.1016/j.phcgj.2013.07.005

[2] Quantitation of oleanolic acid in Oldeniandia corymbosa L. whole-plant powder by high-performance thin-layer chromatography [J].

Banerjee, A ;

T Sane, R ;

Mangaonkar, K ;

Shailajan, S ;

Deshpande, A ;

Gundi, G .

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2006, 19 (107) :68-72

[3] Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects [J].

Banno, N ;

Akihisa, T ;

Tokuda, H ;

Yasukawa, K ;

Higashihara, H ;

Ukiya, M ;

Watanabe, K ;

Kimura, Y ;

Hasegawa, J ;

Nishino, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2004, 68 (01) :85-90

[4] Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella vulgaris L. by capillary zone electrophoresis with the addition of running buffer modifiers [J].

Cheung, Hon-Yeung ;

Zhang, Qing-Feng .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1213 (02) :231-238

[5] Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer [J].

Claude, Berengere ;

Morin, Philippe ;

Lafosse, Michel ;

Belmont, Anne-Sophie ;

Haupt, Karsten .

TALANTA, 2008, 75 (02) :344-350

[6] 6-Oxy-(acetyl piperazine) fluorescein as a new fluorescent labeling reagent for free fatty acids in serum using high-performance liquid chromatography [J].

Du, Xiao-Lan ;

Zhang, Hua-Shan ;

Guo, Xiao-Feng ;

Deng, Ying-Hua ;

Wang, Hong .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1169 (1-2) :77-85

[7] Isolation of ursolic acid from apple peels by high speed counter-current chromatography [J].

Frighetto, Rosa T. S. ;

Welendorf, Rodolfo M. ;

Nigro, Eduardo N. ;

Frighetto, Nelson ;

Siani, Antonio C. .

FOOD CHEMISTRY, 2008, 106 (02) :767-771

[8] HPLC quantification of two isomeric triterpenic acids isolated from Mitracarpus scaber and antimicrobial activity on Dermatophilus congolensis [J].

Gbaguidi, F ;

Accrombessi, G ;

Moudachirou, M ;

Quetin-Leclercq, J .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 39 (05) :990-995

[9] Characterization of Triterpenic Acids in Fruits of Ziziphus Species by HPLC-ELSD-MS [J].

Guo, Sheng ;

Duan, Jin-Ao ;

Tang, Yu-Ping ;

Yang, Nian-Yun ;

Qian, Da-Wei ;

Su, Shu-Lan ;

Shang, Er-Xin .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (10) :6285-6289

[10] DLLME Combined with GC-MS for the Determination of Methylparaben, Ethylparaben, Propylparaben and Butylparaben in Beverage Samples [J].

Han, Yi ;

Jia, Xiaoyu ;

Liu, Xinli ;

Duan, Taicheng ;

Chen, Hangting .

CHROMATOGRAPHIA, 2010, 72 (3-4) :351-355

← 1 2 3 4 →