Multiplex Digital Polymerase Chain Reaction Technology and Its Application

被引:1
作者
Yin Ju-Xin [1 ,2 ]
Xia Li-Ping [2 ]
Zou Zhe-Yu [2 ]
Mu Ying [2 ]
机构
[1] Zhejiang Univ City Coll, Sch Informat & Elect Engn, Hangzhou 310015, Peoples R China
[2] Zhejiang Univ, Res Ctr Analyt Instrumentat, State Key Lab Ind Control Technol, Hangzhou 310027, Peoples R China
基金
中国国家自然科学基金;
关键词
Digital polymerase chain reaction; Microfluidics; Multiplex detection; Review; GUIDELINES MINIMUM INFORMATION; ABSOLUTE QUANTIFICATION; QUANTITATIVE ASSESSMENT; MICROFLUIDIC CHIP; SELF-DIGITIZATION; ANALYSIS SYSTEMS; PCR; DNA; GENERATION; AMPLIFICATION;
D O I
10.19756/j.issn.0253-3820.210530
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Digital polymerase chain reaction (PCR), as a nucleic acid detection technology with wide application prospect, has become one of the most accurate nucleic acid detection technology at present. Multiplex detection is an important direction for the development of digital PCR technique. With the development of microfluidic technology, multiplex digital PCR technique has become more and more mature. This paper reviewed the research progresses of multiplex digital PCR in recent years, especially summarized the implementation of multiplex digital PCR technique in the past five years, and introduced the application of multiplex digital PCR technique in hot areas such as liquid biopsy, transgenic detection, and SARS-Cov-2 detection. Finally, the issues and challenges faced by multiplex digital PCR technique were discussed and the future direction of the technology was foreseen.
引用
收藏
页码:25 / 38
页数:14
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