A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

被引：7

作者：

Coban, Ahmet Yilmaz ^{[1
]}

Cayci, Yeliz Tanriverdi ^{[1
]}

Deveci, Aydin ^{[2
]}

Akgunes, Alper ^{[3
]}

Uzun, Meltem ^{[4
]}

Durupinar, Belma ^{[1
]}

机构：

[1] Ondokuz Mayis Univ, Sch Med, Dept Med Microbiol, Samsun, Turkey

[2] Ondokuz Mayis Univ, Sch Med, Dept Clin Microbiol & Infect Dis, Samsun, Turkey

[3] Samsun Chest Dis Hosp, Clin Microbiol Lab, Dept Microbiol & Clin Microbiol, Samsun, Turkey

[4] Istanbul Univ, Istanbul Med Sch, Istanbul, Turkey

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2011年 / 106卷 / 03期

关键词：

Mycobacterium tuberculosis; nitrate reductase assay; blood agar; COLORIMETRIC METHOD; LIQUID CULTURES; SPUTUM SAMPLES; SUSCEPTIBILITY; RESAZURIN; RIFAMPICIN; DRUGS;

D O I：

10.1590/S0074-02762011000300022

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.

引用

页码：378 / 380

页数：3

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