Anti-obesity effect of carboxymethyl chitin by AMPK and aquaporin-7 pathways in 3T3-L1 adipocytes

被引：27
作者：
Kong, Chang-Suk [2 ]
Kim, Jung-Ae [1 ]
Bak, Soon-Sun [3 ]
Byun, Hee-Guk [4 ]
Kim, Se-Kwon [1 ,3 ]
机构：
[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea
[2] Silla Univ, Dept Food & Nutr, Coll MEd & Life Sci, Pusan 617736, South Korea
[3] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea
[4] Kangnung Natl Univ, Fac Sci & Biotechnol, Kangnung 210702, South Korea
来源：
JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 2011年 / 22卷 / 03期
关键词：
Adipocyte differentiation; Adipogenesis; AMPK; Aquaporin-7; Lipid accumulation; ACTIVATED PROTEIN-KINASE; CHITOSAN; ADIPOSE; OBESITY; LIPOLYSIS;
D O I：
10.1016/j.jnutbio.2010.02.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study was to investigate the anti-obesity effect of carboxymethyl-chitin (CM-chitin). a water-soluble derivative of chitin, by measuring lipid accumulation and adipogenesis related factors in 3T3-L1 adipocytes. CM-chitin was synthesized by means of carboxymethylation reaction. Its inhibitory effect on lipid accumulation was investigated by measuring triglyceride content and glycerol release level. The gene and protein levels associated with adipogenesis were determined using reverse transcriptase-polymerase chain reaction and Western blot analysis. Treatment with CM-chitin reduced triglyceride content and enhanced glycerol secretion in a dose-dependent manner. CM-chitin induced the down-regulation of adipogenesis related transcriptional factors and adipocyte specific gene promoters. Moreover, the specific mechanism by CM-chitin was confirmed by transcriptional activations of the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and aquaporin-7. These results suggest that CM-chitin exerts anti-adipogenic effect on lipid accumulation through modulations of AMPK and aquaporin-7 signal pathways. (C) 2011 Elsevier Inc. All rights reserved.
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收藏
页码：276 / 281
页数：6
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