SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL

被引：36

作者：

Yvan-Charvet, Laurent ^{[1
]}

Pagler, Tamara A. ^{[1
]}

Wang, Nan ^{[1
]}

Senokuchi, Takafumi ^{[1
]}

Brundert, May ^{[2
]}

Li, Hongna ^{[1
]}

Rinninger, Franz

Tall, Alan R. ^{[1
]}

机构：

[1] Columbia Univ, Dept Med, Div Mol Med, New York, NY 10027 USA

[2] Univ Hamburg Hosp, Hamburg, Germany

来源：

JOURNAL OF LIPID RESEARCH | 2008年 / 49卷 / 01期

关键词：

high density lipoprotein; ATP binding cassette transporter G1; scavenger receptor class B type I; lecithin : cholesterol acyltransferase; liver X receptor; macrophage;

D O I：

10.1194/jlr.M700200-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study compares the roles of ABCG1 and scavenger receptor class B type I (SR-BI) singly or together in promoting net cellular cholesterol efflux to plasma HDL containing active LCAT. In transfected cells, SR-BI promoted free cholesterol efflux to HDL, but this was offset by an increased uptake of HDL cholesteryl ester (CE) into cells, resulting in no net efflux. Coexpression of SR-BI with ABCG1 inhibited the ABCG1-mediated net cholesterol efflux to HDL, apparently by promoting the reuptake of CE from medium. However, ABCG1-mediated cholesterol efflux was not altered in cholesterol-loaded, SR-BI-deficient (SR-BI-/-) macrophages. Briefly cultured macrophages collected from SR-BI-/- mice loaded with acetylated LDL in the peritoneal cavity did exhibit reduced efflux to HDL. However, this was attributable to reduced expression of ABCG1 and ABCA1, likely reflecting increased macrophage cholesterol efflux to apolipoprotein E-enriched HDL during loading in SR-BI-/- mice.

引用

页码：107 / 114

页数：8

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