Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

被引：12

作者：

Elfaramawy, Maie A. ^{[1
]}

Fujii, Satoshi ^{[2
]}

Uyeda, Atsuko ^{[1
]}

Osaki, Toshihisa ^{[2
,3
]}

Takeuchi, Shoji ^{[2
,3
]}

Kato, Yasuhiko ^{[1
]}

Watanabe, Hajime ^{[1
]}

Matsuura, Tomoaki ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Div Adv Sci & Biotechnol, Dept Biotechnol, 2-1Yamadaoka, Suita, Osaka 5650871, Japan

[2] Kanagawa Inst Ind Sci & Technol, Artificial Cell Membrane Syst Grp, Takatsu Ku, 3-2-1 Sakado, Kawasaki, Kanagawa 2130012, Japan

[3] Univ Tokyo, Inst Ind Sci, Meguro Ku, 4-6-1 Komaba, Tokyo 1538505, Japan

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 86期

基金：

日本学术振兴会;

关键词：

IN-VITRO SYNTHESIS; MULTIDRUG TRANSPORTER; ALPHA-HEMOLYSIN; SEC TRANSLOCON; BINDING; COLI; EMRE; CONTACT; CHANNEL; PORES;

D O I：

10.1039/c8cc06804f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the functional synthesis and quantification of membrane proteins-alpha-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli-at the stabilized droplet interface bilayer using an in vitro transcription-translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.

引用

页码：12226 / 12229

页数：4

共 49 条

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