Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

被引：12
|
作者：
Elfaramawy, Maie A. [1 ]
Fujii, Satoshi [2 ]
Uyeda, Atsuko [1 ]
Osaki, Toshihisa [2 ,3 ]
Takeuchi, Shoji [2 ,3 ]
Kato, Yasuhiko [1 ]
Watanabe, Hajime [1 ]
Matsuura, Tomoaki [1 ]
机构：
[1] Osaka Univ, Grad Sch Engn, Div Adv Sci & Biotechnol, Dept Biotechnol, 2-1Yamadaoka, Suita, Osaka 5650871, Japan
[2] Kanagawa Inst Ind Sci & Technol, Artificial Cell Membrane Syst Grp, Takatsu Ku, 3-2-1 Sakado, Kawasaki, Kanagawa 2130012, Japan
[3] Univ Tokyo, Inst Ind Sci, Meguro Ku, 4-6-1 Komaba, Tokyo 1538505, Japan
来源：
CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 86期
基金：
日本学术振兴会;
关键词：
IN-VITRO SYNTHESIS; MULTIDRUG TRANSPORTER; ALPHA-HEMOLYSIN; SEC TRANSLOCON; BINDING; COLI; EMRE; CONTACT; CHANNEL; PORES;
D O I：
10.1039/c8cc06804f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the functional synthesis and quantification of membrane proteins-alpha-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli-at the stabilized droplet interface bilayer using an in vitro transcription-translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.
引用
收藏
页码：12226 / 12229
页数：4
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