LncRNA PSMA3-AS1 promotes cell proliferation, migration, and invasion in ovarian cancer by activating the PI3K/Akt pathway via the miR-378a-3p/GALNT3 axis

被引：20

作者：

Xu, Zhihong ^{[1
]}

Jin, Hui ^{[1
]}

Duan, Xiaoyang ^{[1
]}

Liu, Hong ^{[2
]}

Zhao, Xiwa ^{[2
]}

Fan, Shaoshuang ^{[1
]}

Wang, Yan ^{[3
]}

Yao, Tiezhu ^{[4
]}

机构：

[1] Hebei Med Univ, Dept Oncol, Hosp 4, Shijiazhuang, Hebei, Peoples R China

[2] Hebei Med Univ, Dept Gynecol, Hosp 4, Shijiazhuang, Hebei, Peoples R China

[3] Hebei Med Univ, Dept Thorac Surg, Hosp 4, Shijiazhuang, Hebei, Peoples R China

[4] Hebei Med Univ, Dept Cardiol, Hosp 4, Shijiazhuang, Hebei, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY | 2021年 / 36卷 / 12期

关键词：

GALNT3; miR-378a-3p; ovarian cancer; PI3K; Akt pathway; PSMA3-AS1; LONG NONCODING RNA; EXPRESSION; PROGRESSION; PROGNOSIS; REVEALS; GROWTH;

D O I：

10.1002/tox.23370

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The crucial roles of the long noncoding RNAs (lncRNAs) in the development of ovarian cancer (OC) have been extensively studied. According to the prediction result from the Kaplan-Meier Plotter database, high expression of lncRNA proteasome subunit alpha type-3 antisense RNA1 (PSMA3-AS1) is associated with the poor prognosis in patients with OC. Thus, the study aimed to investigate the role of lncRNA PSMA3-AS1 in OC. Reverse transcription quantitative polymerase chain reaction analysis revealed that PSMA3-AS1 expression was significantly upregulated in OC cells and tissues. PSMA3-AS1 silencing inhibited OC cell proliferation, migration, and invasion, as shown by results of cell counting kit-8, colony formation, wound healing, and Transwell assays, respectively. Additionally, PSMA3-AS1 deficiency suppressed tumor growth in vivo. Mechanistically, luciferase reporter and RNA pulldown assays implied that PSMA3-AS1 served as a competing endogenous RNA for miR-378a-3p to upregulate the expression of polypeptide N-acetylgalactosaminyltransferase 3 (GALNT3). GALNT3 was a target gene of miR-378a-3p in OC. Moreover, PSMA3-AS1 activated the PI3K/Akt pathway by upregulating GALNT3 expression. Overall, PSMA3-AS1 promotes OC cell proliferation, migration, invasion, and xenograft tumor growth by activating the PI3K/Akt pathway via the miR-378a-3p/GALNT3 axis.

引用

页码：2562 / 2577

页数：16

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