Differential regenerative capacity of neonatal mouse hearts after cryoinjury

被引：81

作者：

Darehzereshki, Ali ^{[1
,2
]}

Rubin, Nicole ^{[1
,2
]}

Gamba, Laurent ^{[1
,2
]}

Kim, Jieun ^{[1
,2
]}

Fraser, James ^{[1
,2
]}

Huang, Ying ^{[1
,2
]}

Billings, Joshua ^{[1
,2
]}

Mohammadzadeh, Robabeh ^{[3
]}

Wood, John ^{[1
,2
,4
]}

Warburton, David ^{[2
,4
]}

Kaartinen, Vesa ^{[5
]}

Lien, Ching-Ling ^{[1
,2
,6
]}

机构：

[1] Hosp Good Samaritan, Inst Heart, Los Angeles, CA 90017 USA

[2] Childrens Hosp Los Angeles, Saban Res Inst, Los Angeles, CA 90027 USA

[3] Univ So Calif, Broad Ctr Stem Cell & Regenerat Med, Los Angeles, CA 90027 USA

[4] Univ So Calif, Keck Sch Med, Dept Pediat, Los Angeles, CA 90027 USA

[5] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA

[6] Univ So Calif, Keck Sch Med, Dept Surg Biochem & Mol Biol, Los Angeles, CA 90027 USA

来源：

DEVELOPMENTAL BIOLOGY | 2015年 / 399卷 / 01期

关键词：

Neonatal mouse heart regeneration; Cryoinjury; Cardiomyocyte proliferation; Epicardium; Neovascularization; PLASMINOGEN-ACTIVATOR INHIBITOR-1; TO-MESENCHYMAL TRANSITION; MYOCARDIAL-INFARCTION; CARDIAC FIBROSIS; APEX RESECTION; ZEBRAFISH; ADULT; CARDIOMYOCYTES; CONTRIBUTES; DEFICIENCY;

D O I：

10.1016/j.ydbio.2014.12.018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Neonatal mouse hearts fully regenerate after ventricular resection similar to adult zebrafish. We established cryoinjury models to determine if different types and varying degrees of severity in cardiac injuries trigger different responses in neonatal mouse hearts. In contrast to ventricular resection, neonatal mouse hearts fail to regenerate and show severe impairment of cardiac function post transmural cryoinjury. However, neonatal hearts fully recover after non-transmural cryoinjury. Interestingly, cardiomyocyte proliferation does not significantly increase in neonatal mouse hearts after cryoinjuries. Epicardial activation and new coronary vessel formation occur after cryoinjury. The profibrotic marker PAI-1 is highly expressed after transmural but not non-transmural cryoinjuries, which may contribute to the differential scarring. Our results suggest that regenerative medicine strategies for heart injuries should vary depending on the nature of the injury. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：91 / 99

页数：9

共 33 条

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