CDK12 Activity-Dependent Phosphorylation Events in Human Cells

被引：10

作者：

Bartkowiak, Bartlomiej ^{[1
]}

Yan, Christopher M. ^{[1
]}

Soderblom, Erik J. ^{[2
]}

Greenleaf, Arno L. ^{[1
]}

机构：

[1] Duke Med Ctr, Dept Biochem, Durham, NC 27710 USA

[2] Duke Med Ctr, Prote Core Facil, Durham, NC 27710 USA

来源：

BIOMOLECULES | 2019年 / 9卷 / 10期

基金：

美国国家卫生研究院;

关键词：

analog-sensitive CTD kinase; transcription; CTD; RNA polymerase II; RNA processing; mRNA nuclear export; nuclear pore; mESC undifferentiated state; XPC; TPR; 1-NM-PP1; C-TERMINAL DOMAIN; RNA-POLYMERASE-II; PRE-MESSENGER-RNA; KINASE CTDK-I; REPEAT DOMAIN; PROTEIN-KINASE; YEAST; TRANSCRIPTION; COMPLEX; EXPRESSION;

D O I：

10.3390/biom9100634

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We asked whether the C-terminal repeat domain (CTD) kinase, CDK12/CyclinK, phosphorylates substrates in addition to the CTD of RPB1, using our CDK12(analog-sensitive) HeLa cell line to investigate CDK12 activity-dependent phosphorylation events in human cells. Characterizing the phospho-proteome before and after selective inhibition of CDK12 activity by the analog 1-NM-PP1, we identified 5,644 distinct phospho-peptides, among which were 50 whose average relative amount decreased more than 2-fold after 30 min of inhibition (none of these derived from RPB1). Half of the phospho-peptides actually showed >3-fold decreases, and a dozen showed decreases of 5-fold or more. As might be expected, the 40 proteins that gave rise to the 50 affected phospho-peptides mostly function in processes that have been linked to CDK12, such as transcription and RNA processing. However, the results also suggest roles for CDK12 in other events, notably mRNA nuclear export, cell differentiation and mitosis. While a number of the more-affected sites resemble the CTD in amino acid sequence and are likely direct CDK12 substrates, other highly-affected sites are not CTD-like, and their decreased phosphorylation may be a secondary (downstream) effect of CDK12 inhibition.

引用

页数：14

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