Affinity dependence of the B cell response to antigen: A threshold, a ceiling, and the importance of off-rate

被引：371

作者：

Batista, FD ^{[1
]}

Neuberger, MS ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

IMMUNITY | 1998年 / 8卷 / 06期

关键词：

D O I：

10.1016/S1074-7613(00)80580-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Initiation and affinity maturation of the humoral immune response is driven by antigen interaction with BCR. To study how signaling and antigen presentation through BCR depend on antigen/BCR affinity, lysozyme-specific B cell transfectants were challenged with mutated lysozymes differing in their binding kinetics. For detectable triggering, the antigen/BCR complex needed a K-a > 10(6) M-1 (dissociation half-life > similar to 1 s). Mutated lysozymes whose binding was below this threshold could nevertheless be presented if complexed with soluble antibody. Above the threshold, the concentration of antigen required to trigger a response decreased as the affinity (particularly dissociation half-life) increased. However, a plateau was reached at K(a)s > similar to 10(10) M-1 (dissociation half-life > 0.5 hr), supporting the idea of a ceiling to affinity maturation.

引用

页码：751 / 759

页数：9

共 42 条

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