Anti-inflammatory action of Pluchea sagittalis: Involvement of an antioxidant mechanism

被引：32

作者：

PerezGarcia, F ^{[1
]}

Marin, E ^{[1
]}

Canigueral, S ^{[1
]}

Adzet, T ^{[1
]}

机构：

[1] UNIV BARCELONA, FAC FARM, UNITAT FARMACOL & FARMACOGNOSIA, E-08028 BARCELONA, SPAIN

来源：

LIFE SCIENCES | 1996年 / 59卷 / 24期

关键词：

anti-inflammatory; antioxidant; Pluchea sagittalis;

D O I：

10.1016/S0024-3205(96)00556-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Pluchea sagittalis (Lam.) Cabr., a popular medicinal herb grown in South America, was studied for anti-inflammatory and antioxidant activities. The anti-edema action of P. sagittalis aqueous extract was assayed in different models of inflammation: 1) the mouse ear edema test induced by arachidonic acid and croton oil; 2) the rat hind-paw edema test produced by several inflammatory inductors: carrageenan, dextran, zymosan, platelet-activating factor (PAF) and arachidonic acid; 3) a subacute model based on the rat carrageenan air-pouch granuloma test. Blood leukocyte free radical production was measured by flow cytometry with 2',7'-dichlorofluorescin diacetate (DCFH-DA) in vivo, in rats with induced air-pouch granuloma, and in a model in vitro, stimulating leukocytes with hydrogen peroxide. The aqueous extract of P. sagittalis showed a marked anti-inflammatory effect in both ear edema tests, dextran and carrageenan hind-paw edemas and carrageenan air-pouch model. It also had a potent antioxidant activity in blood leukocytes, both in vivo and in vitro. Our results correlate the reduction of free radical production with the anti-inflammatory effect of this plant.

引用

页码：2033 / 2040

页数：8

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