Effects of Polyhydroxy Compounds on Enzymatic Synthesis of L-Tryptophan Catalyzed by Tryptophan Synthase

被引：2

作者：

Xu, Lisheng ^{[1
,2
]}

Wang, Zhiyuan ^{[1
]}

Liu, Junzhong ^{[1
]}

Mao, Pingting ^{[1
]}

Zhang, Hongjuan ^{[1
]}

Gao, Ji ^{[1
]}

Liu, Qian ^{[1
]}

Jiao, Qingcai ^{[1
]}

机构：

[1] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

[2] Suzhou Univ, Dept Chem & Life Sci, Suzhou 234000, Peoples R China

来源：

CATALYSIS LETTERS | 2012年 / 142卷 / 02期

关键词：

Tryptophan synthase; Polyethylene glycol; Additives; Kinetic parameters; ADDITIVES; THERMOSTABILITY; ALCOHOLS;

D O I：

10.1007/s10562-011-0743-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The effects of polyethylene glycol (PEG) of different molecular (1000, 2000), glycerol, ethylene glycol on the catalytic activity of tryptophan synthase were studied. The results indicated that the addition of PEG 2000 increased the enzymatic activity of tryptophan synthase. The enzymatic activity of tryptophan synthase was enhanced 25.2% by 10 g L-1 PEG 2000. Reaction conditions were optimized by using 10 g L-1 PEG 2000 at pH 9 and 40 A degrees C. L-Serine conversion rate reach 89.9% under the optimal conditions. The kinetic parameters indicated the specificity of TSase to substrate was improved.

引用

页码：282 / 286

页数：5

共 19 条

[1]

Alam MN, 1996, BIOTECHNOL LETT, V18, P45

[2] Effect of soluble additives on enzyme thermo- and/or baro-deactivation [J].

Athès, V ;

Guerra, P ;

Combes, D .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1999, 7 (1-4) :1-9

[3] PRODUCTION OF L-TRYPTOPHAN BY ESCHERICHIA-COLI-CELLS [J].

BANG, WG ;

LANG, S ;

SAHM, H ;

WAGNER, F .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (04) :999-1011

[4] INTERACTION OF ALCOHOLS WITH PROTEINS [J].

BULL, HB ;

BREESE, K .

BIOPOLYMERS, 1978, 17 (09) :2121-2131

[5] STABILIZING EFFECT OF POLYHYDRIC ALCOHOLS - INFLUENCE OF THE ENZYME [J].

COMBES, D ;

GRABER, M ;

YE, WN .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1990, 613 :559-563

[6] Prolonged production of tryptophan using immobilized bacteria [J].

Dallmann, K ;

Orosz, LS ;

Szajani, B .

BIOTECHNOLOGY LETTERS, 1997, 19 (02) :123-125

[7] Tryptophan Synthase: Structure and Function of the Monovalent Cation Site [J].

Dierkers, Adam T. ;

Niks, Dimitri ;

Schlichting, Ilme ;

Dunn, Michael F. .

BIOCHEMISTRY, 2009, 48 (46) :10997-11010

[8] Ligand-Induced Formation of a Transient Tryptophan Synthase Complex with αββ Subunit Stoichiometry [J].

Ehrmann, Alexander ;

Richter, Klaus ;

Busch, Florian ;

Reimann, Julia ;

Albers, Sonja-Verena ;

Sterner, Reinhard .

BIOCHEMISTRY, 2010, 49 (51) :10842-10853

[9] A convenient enzymatic synthesis of L-halotryptophans [J].

Goss, Rebecca J. M. ;

Newill, Philip L. A. .

CHEMICAL COMMUNICATIONS, 2006, (47) :4924-4925

[10] CONTRIBUTION OF THE SURFACE FREE-ENERGY PERTURBATION TO PROTEIN SOLVENT INTERACTIONS [J].

KITA, Y ;

ARAKAWA, T ;

LIN, TY ;

TIMASHEFF, SN .

BIOCHEMISTRY, 1994, 33 (50) :15178-15189

← 1 2 →