Genome-wide identification and expression pattern analysis of novel chemosensory genes in the German cockroach Blattella germanica

被引：11

作者：

He, Ming ^{[1
]}

Ma, Yun-Feng ^{[1
]}

Guo, Huan ^{[1
]}

Liu, Xuan-Zheng ^{[1
]}

Long, Gui-Jun ^{[1
]}

Wang, Qin ^{[1
]}

Dewer, Youssef ^{[2
]}

Zhang, Fan ^{[3
]}

He, Peng ^{[1
]}

机构：

[1] Guizhou Univ, Minist Educ, Key Lab Green Pesticide & Agr Bioengn, State Key Lab Breeding Base Green Pesticide & Agr, Guiyang 550025, Peoples R China

[2] Agr Res Ctr, Phytotox Res Dept, Cent Agr Pesticide Lab, 7 Nadi El Seid St, Giza 12618, Egypt

[3] Shandong Normal Univ, Coll Life Sci, Key Lab Anim Resistance Res, 88 East Wenhua Rd, Jinan 250014, Peoples R China

来源：

GENOMICS | 2022年 / 114卷 / 02期

基金：

中国国家自然科学基金;

关键词：

German cockroach; Chemosensory proteins; Sensory neuron membrane proteins; Gustatory receptors; Ionotropic receptors; ODORANT-BINDING-PROTEINS; IONOTROPIC GLUTAMATE RECEPTORS; CONTACT SEX-PHEROMONE; MOLECULAR-BASIS; IMMUNOCYTOCHEMICAL LOCALIZATION; SOLUBLE-PROTEINS; FAMILY; TASTE; PERCEPTION; ECOLOGY;

D O I：

10.1016/j.ygeno.2022.110310

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The German cockroach Blattella germanica is an important urban insect pest worldwide. In many insects, chemosensation is essential for guiding their behaviors for survival. Although a large number of chemosensoryrelated genes have been identified in B. germanica, little information on tissue-specific and developmental expression patterns has not been uncovered yet. In this study, we performed transcriptome analysis of different B. germanica tissues to reveal novel chemosensory proteins (CSPs) and sensory neuron membrane proteins (SNMPs). In addition, a phylogenetic tree and gender-specific expression of multiple chemosensory gene families have been analyzed. We identified three CSPs genes (BgerCSP11, BgerCSP12, and BgerCSP13) and five SNMP genes in B. germanica. Tissue-specific expression profiling showed that CSP1, 8, and 9 exhibited significant expression levels in both adult and 5th instar nymph antennae. The results have paved the way for further functional study of the chemosensory mechanism in B. germanica and provided potential insecticide targets.

引用

页数：11

共 114 条

[1] Ionotropic Glutamate Receptors IR64a and IR8a Form a Functional Odorant Receptor Complex In Vivo in Drosophila [J].