Refractive index measurements of microvolumes liquids with control of the temperature

In this work an automatic operating prototype of a laser refractometer is described. The device consists of a refractometric block, where the refractive index (RI) of liquids is detected according to modified prism method, build-in interferometer for determination of the angle of rotation, which use the same laser and system for contact measurement and control of the liquid temperature with an accuracy of 0.3 degreesC. The automatization of the measurement avoid the subjective related errors. The construction permits the utilization of various lasers as light sources. The achieved accuracy of about 1.10(-4) allows applications of the refractometer in various kinds of problems as optical constant determination concentration measurements, purity control, in situ biomedicine diagnostic and etc.