Production and characterisation of monoclonal antibodies specific for bovine interleukin-4

被引：15

作者：

Weynants, V

Gilson, D

Furger, A

Collins, RA

Mertens, P

De Bolle, X

Heussler, VT

Roditi, I

Howard, CJ

Dobbelaere, AE

Letesson, JJ

机构：

[1] Fac Univ Notre Dame Paix, Unite Immunol Microbiol, B-5000 Namur, Belgium

[2] Univ Bern, Inst Gen Microbiol, CH-3012 Bern, Switzerland

[3] AFRC, Inst Anim Hlth, Newbury RG16 0NN, Berks, England

[4] Univ Bern, Inst Anim Pathol, Bern, Switzerland

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1998年 / 66卷 / 02期

关键词：

bovine; interleukin-4; monoclonal antibodies; intracellular staining; flow cytometry;

D O I：

10.1016/S0165-2427(98)00184-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Genetic immunisation is a simple method for producing polyclonal antibodies in mice. By this method, we produced antibodies against bovine interleukin-4 (BoIL-4). After a final injection with a recombinant BoIL-4 protein, nine stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of each of the MAbs to recombinant BoIL-4 produced by Escherichia coli, baculovirus, and Trypanosoma brucei was demonstrated in an indirect ELISA and/or in Western blotting. These MAbs recognise the same antigenic region localised in the first 47 amino acids of the mature protein. None of them was able to neutralise the biological activity of the BoIL-4 under the conditions tested but one allowed the detection of BolL-4 by flow cytometry. (C) 1998 Elsevier Science B.V, All rights reserved.

引用

页码：99 / 112

页数：14

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