Cdc42/N-WASP and Rac1/WAVE2 Required for LPA-induced Migration of Rat Ascites Hepatoma Cells

被引：1

作者：

Uchiyama, Ayako ^{[1
]}

Tigyi, Gabor ^{[2
]}

Murakami-Murofushi, Kimiko ^{[1
]}

机构：

[1] Ochanomizu Univ, Grad Sch Humanities & Sci, Bunkyo Ku, Tokyo 1128610, Japan

[2] Univ Tennessee, Hlth Sci Ctr, Dept Physiol, Memphis, TN 38163 USA

来源：

CYTOLOGIA | 2010年 / 75卷 / 02期

关键词：

Lysophosphatidic acid; LPA; Tumor cell invasion; Rho family GTPases; ACTIN STRESS FIBERS; RHO-GTPASES; LYSOPHOSPHATIDIC ACID; TUMOR-CELLS; INVASION; ADHESION; KINASE; CDC42; POLARITY; BIOLOGY;

D O I：

10.1508/cytologia.75.195

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Lysophosphatidic acid (LPA) has been shown to stimulate transcellular migration of MM I rat ascites hepatoma cells through transient activation of the Rho/ROCK pathway. Here we report that LPA also induces transient inactivation of Cdc42 and Rac1, both of which are required for transmigration of MM1 cells. Cdc42-induced shape change is a required step for LPA-induced MM1 cell migration. We provide evidence that MM1 cells transmigrate the mesothelial cell monolayer through activation of not only previously reported Rho/ROCK pathway but also Rac/WAVE2 and Cdc42/N-WASP pathways.

引用

页码：195 / 201

页数：7

共 26 条

[1]

AKEDO H, 1986, CANCER RES, V46, P2416

[2] Cooperation of fibronectin with lysophosphatidic acid induces motility and transcellular migration of rat ascites hepatoma cells [J].

Ayaki, M ;

Mukai, M ;

Imamura, F ;

Iwasaki, T ;

Mammoto, T ;

Shinkai, K ;

Nakamura, H ;

Akedo, H .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2000, 1495 (01) :40-50

[3] Rho GTPases: functions and association with cancer [J].

Ellenbroek, Saskia I. J. ;

Collard, John G. .

CLINICAL & EXPERIMENTAL METASTASIS, 2007, 24 (08) :657-672

[4] Cdc42 - the centre of polarity [J].

Etienne-Manneville, S .

JOURNAL OF CELL SCIENCE, 2004, 117 (08) :1291-1300

[5] Rho GTPases in cell biology [J].

Etienne-Manneville, S ;

Hall, A .

NATURE, 2002, 420 (6916) :629-635

[6] Roles of Rho-family GTPases in cell polarisation and directional migration [J].

Fukata, M ;

Nakagawa, M ;

Kaibuchi, K .

CURRENT OPINION IN CELL BIOLOGY, 2003, 15 (05) :590-597

[7] SERUM REQUIREMENT FOR INVITRO INVASION BY TUMOR-CELLS [J].

IMAMURA, F ;

HORAI, T ;

MUKAI, M ;

SHINKAI, K ;

AKEDO, H .

JAPANESE JOURNAL OF CANCER RESEARCH, 1991, 82 (05) :493-496

[8] Involvement of small GTPases Rho and Rac in the invasion of rat ascites hepatoma cells [J].

Imamura, F ;

Mukai, M ;

Ayaki, M ;

Takemura, K ;

Horai, T ;

Shinkai, K ;

Nakamura, H ;

Akedo, H .

CLINICAL & EXPERIMENTAL METASTASIS, 1999, 17 (02) :141-148

[9] Y-27632, an inhibitor of Rho-associated protein kinase, suppresses tumor cell invasion via regulation of focal adhesion and focal adhesion kinase [J].

Imamura, F ;

Mukai, M ;

Ayaki, M ;

Akedo, H .

JAPANESE JOURNAL OF CANCER RESEARCH, 2000, 91 (08) :811-816

[10] INDUCTION OF INVITRO TUMOR-CELL INVASION OF CELLULAR MONOLAYERS BY LYSOPHOSPHATIDIC ACID OR PHOSPHOLIPASE-D [J].

IMAMURA, F ;

HORAI, T ;

MUKAI, M ;

SHINKAI, K ;

SAWADA, M ;

AKEDO, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 193 (02) :497-503

← 1 2 3 →