Structures and properties of the blend membrane of regenerated silk fibroin and poly(vinyl alcohol) and its application to glucose sensor

The morphologies and structures of the blend membranes of regenerated silk fibroin and poly(vinyl alcohol) were investigated with scanning electron microscopy, and their water absorbility and mechanical properties were measured, The amperometric p-benzoquinon mediating sensor for glucose based on glucose oxidase immobilized in the blend membrane of regenerated silk fibroin and poly(vinyl alcohol) possessed perfect stability and high sensitivity, and its linear range was from 10(-5) to 10(-2) mol/L with the response time less than 20 s, Moreover, its lifetime was over two months.