Cluster specific regulation pattern of upstream regulatory elements in human α- and β-globin gene clusters

被引：2

作者：

Tang, Yi ^{[1
]}

Huang, Yue ^{[1
]}

Shen, Wei ^{[1
]}

Liu, Guang ^{[1
]}

Wang, Zhao ^{[1
]}

Tan, Xiao-bin ^{[1
]}

Feng, Dong-xiao ^{[1
]}

Liu, De-pei ^{[1
]}

Liang, Chih-chuan ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2008年 / 314卷 / 01期

基金：

中国国家自然科学基金;

关键词：

human globin genes family; upstream regulatory element; expression regulation; transgenic mice; bacterial artificial chromosome;

D O I：

10.1016/j.yexcr.2007.08.014

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Located in different chromatin contexts and with different developmental switching mode, human alpha- and beta-globin gene clusters are co-regulated temporally and quantitatively to keep balanced expression. Here, by exchanging their key upstream regulatory elements (UREs) in cluster level, and investigating the expression level of exogenous globin genes in the bacterial artificial chromosome (BAC) mediated transgenic mice, we explored the similarities and differences in the regulatory effects between a-upstream regulatory element (alpha-URE) and beta-locus control region (beta-LCR). The results showed that, after exchange, the developmental switching modes of human alpha- and beta-like globin genes had changed, with lost expression of epsilon- and alpha 1-genes. Their expression levels also decreased. Our study suggests that the regulation of alpha-URE and beta-LCR on the expression level and developmental switching mode of downstream globin genes is cluster specific. (C) 2007 Published by Elsevier Inc.

引用

页码：115 / 122

页数：8

共 24 条

[1] HUMAN ALPHA-GLOBIN GENES DEMONSTRATE AUTONOMOUS DEVELOPMENTAL REGULATION IN TRANSGENIC MICE [J].