Genetic variation of Indian potato (Solanum tuberosum L.) genotypes using chromosomal and RAPD markers

被引：5

作者：

Das, Anath Bandhu ^{[1
]}

Mohanty, Iswar Chandra ^{[1
]}

Mahapatra, Dawanidhi ^{[1
]}

Mohanty, Suprava ^{[2
]}

Ray, Ashutosh ^{[3
]}

机构：

[1] Orissa Univ Agr & Technol, Coll Agr, Dept Agr Biotechnol, Bhubaneswar 751003, Orissa, India

[2] Reg Plant Resource Ctr, Cytogenet Lab, Bhubaneswar 751015, Orissa, India

[3] Orissa Univ Agr & Technol, Coll Agr, Dept Plant Breeding & Genet, Bhubaneswar 751003, Orissa, India

来源：

CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2010年 / 10卷 / 03期

关键词：

4C DNA content; DNA markers; genotypes; potato; somatic chromosome; NUCLEAR-DNA;

D O I：

10.1590/S1984-70332010000300009

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Genetic diversity in thirty Indian potatoes were analyzed employing karyotype, genome size and RAPD markers. Chromosome analysis revealed 2n = 4x = 48 except cv. K. Chandramukhi (2n = 4x + 2 = 50). Total genomic chromosome length and chromosome volume varied from 21.14 mu m in cc K. Pukhraj to 31.91 mu m in cv. MS/89-60 and 14.31 mu m(3) in cv. MS/92-1090 to 33.16 mu m(3) in cv. JW-160 respectively. 4C DNA content was significantly varied from 3.640 pg (similar to 891 Mbp) in cc MS/92-1090 to 11.12 pg (similar to 2747 Mbp) in cv. K. Chandramukhi. RAPD revealed 131 amplified DNA fragments (300 to 2200 bp) with 79 unique bands (7 to 71% polymorphism) among the genotypes. Similarity coefficients (ranged from 0.29 to 0.93) and cluster analysis reflected the expected trends in relationships of the full and half-sib potato genotypes. Genetic distances obtained from a dendrogram could help breeders to choose the diverse parents for a breeding program aimed at varietal improvement.

引用

页码：238 / 246

页数：9

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