SCoT-marker analysis of Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) and stored date kernels of Phoenix dactylifera (L.) fumigated with ozone and phosphine gases

被引:7
作者
Abdelfattah, Nilly A. H. [1 ]
Al-Qahtani, A. R. [2 ]
Qari, Sameer H. [3 ]
机构
[1] Plant Protect Res Inst, Agr Res Ctr, Cairo, Egypt
[2] Univ Bisha, Coll Sci, Dept Biol, Bisha, Saudi Arabia
[3] Umm Al Qura Univ, Aljumum Univ Coll, Dept Biol, Genet & Mol Biol Cent Lab GMCL, Mecca, Saudi Arabia
关键词
Insecticide; Date palm; DNA-PCR; O; surinamensis; SCOT; Polymorphism; RHYZOPERTHA-DOMINICA; TRIBOLIUM-CASTANEUM; GRAIN BEETLE; RESISTANCE; WHEAT; POLYMORPHISM; SUSCEPTIBILITY; VARIETIES;
D O I
10.1016/j.aspen.2021.07.009
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Oryzaephilus surinamensis, a common grain product pest around the world, particularly causing great damage and losses to the date crops available in the storage. It has been traditionally resisted by using organic pesticides despite suspicions of their harmful effects on dates. This study aimed at the evaluation of the usage of ozone gas as an effective pesticide and also focuses on the safety alternatives provided by the respective method as compared to the usage of phosphine as a pesticide. While studying the ozone effects, the O. surinamensis was exposed to the gas for a variety of time scales (2, 4 and 6 h), distinctly for every life stages of the insect such as egg, larva and adults, infested the Phoenix dactylifera L date palm species present in the Siwa oasis. The obtained results showed that the adult insects had high resistance to ozone gas, while the larvae and egg stages were less resistant. The reduction rate of vital insect was 100% in all stages. Start codon analysis of DNA also showed that there are some SCoT primers can be identified and differentiated between the different treatments and control. On the other hand, the percentage of polymorphisms in insects was 50% while in date kernels 25% by six SCoT primers used. This study highlighted higher efficacy and safety regarding the usage of ozone gas in effectively controlling the manifestation of O. Surinamensis and thereby reducing the loss of stored date crops as compared to the phosphine.
引用
收藏
页码:843 / 849
页数:7
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