Immune Response in Tilapia, Oreochromis niloticus, Induced by the Surface Immunogenic Protein (Sip) of Streptococcus agalactiae

被引：0
作者：
Wang Bei [1 ,2 ,3 ]
Lu Yishan [1 ,2 ,3 ]
Wu Zaohe [2 ,3 ,4 ]
Jian Jichang [1 ,2 ,3 ]
机构：
[1] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China
[2] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China
[3] Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China
[4] Zhongkai Univ Agr & Engn, Guangzhou, Guangdong, Peoples R China
来源：
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH | 2015年 / 67卷
基金：
中国国家自然科学基金;
关键词：
Streptococcus agalactiae; surface immunogenic protein; immunogenicity; vaccine; GROUP-B STREPTOCOCCUS; DOLPHIN TURSIOPS-TRUNCATUS; TOXOID CONJUGATE VACCINE; NONHEMOLYTIC GROUP-B; PROTECTIVE IMMUNITY; EXPERIMENTAL-INFECTION; C-PROTEIN; POLYSACCHARIDE; MICE; IDENTIFICATION;
D O I：
暂无
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The main aim of this study was to demonstrate that the surface immunogenic protein (Sip) of the aquatic pathogen Streptococcus agalactiae is a potential candidate for vaccine development for Oreochromis niloticus. In this study, a Sip was identified. Alignment analysis indicated that the Sip protein was highly homologous with Sip proteins from other S. agalactiae isolated from humans (99%). SDS-PAGE procedure indicated that the Sip protein was successfully expressed in Escherichia coli BL21 (DE3). The recombinant Sip protein was purified by affinity chromatography, and the mouse anti-Sip serum was produced. Tilapia Oreochromis niloticus were vaccinated with Sip, and immunogenicity was confirmed by subsequent western blotting. Enzyme-linked immunosorbent assay (ELISA) analysis demonstrated that Sip produced an observable antibody response in all sera of the vaccinated fish. The relative percentage survival (RPS) value for the Sip vaccine was 90.62. Tilapia vaccinated with Sip produced specific antibodies, and were highly resistant to infection by the virulent S. agalactiae. These results indicate that Sip is an effective vaccine candidate against S. agalactiae for tilapia, Oreochromisniloticus.
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页数：10
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